Gas Chromatography/Mass Spectrometry Analysis: Nontargeted Metabolomics Based on Scan Mode Analysis

H. Tsugawa; A. Lommen

doi:10.1201/b17793-13

Gas Chromatography/Mass Spectrometry Analysis: Nontargeted Metabolomics Based on Scan Mode Analysis

H. Tsugawa, A. Lommen

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Professional

Abstract

Metabolome analysis is divided into two approaches: nontargeted analysis and targeted analysis.1 Nontargeted analysis covers all detected peaks by means of scan mode and utilizes both annotated and unannotated peak information for statistical analyses. This approach has been broadly applied to discovery-phase studies such as biomarker identification for medical

diagnostics. On the other hand, the targeted or widely targeted analysis hunts only “known metabolites” detected by single ion monitoring or selected reaction monitoring mode with the purpose of validating a biological hypothesis or machine-learning studies such as regression and discriminant analyses. In this chapter, we introduce the protocol for the nontargeted gas chromatography/mass spectrometry (GC/MS) based metabolomics.

Original language	English
Title of host publication	Mass Spectrometry-Based Metabolomics
Subtitle of host publication	A Practical Guide
Editors	S. Prama Putri, E. Fukusaki
Place of Publication	Boca Raton
Publisher	CRC Press
Pages	103-136
ISBN (Electronic)	9780429161599
ISBN (Print)	9781482223767, 9781032652245
DOIs	https://doi.org/10.1201/b17793-13
Publication status	Published - 2014

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10.1201/b17793-13

Foodomics metabole profilering (KB-15-001-020)
Lommen, A. (Project Leader)
1/01/12 → 31/12/14
Project: LVVN project

Cite this

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title = "Gas Chromatography/Mass Spectrometry Analysis: Nontargeted Metabolomics Based on Scan Mode Analysis",

abstract = "Metabolome analysis is divided into two approaches: nontargeted analysis and targeted analysis.1 Nontargeted analysis covers all detected peaks by means of scan mode and utilizes both annotated and unannotated peak information for statistical analyses. This approach has been broadly applied to discovery-phase studies such as biomarker identification for medical diagnostics. On the other hand, the targeted or widely targeted analysis hunts only “known metabolites” detected by single ion monitoring or selected reaction monitoring mode with the purpose of validating a biological hypothesis or machine-learning studies such as regression and discriminant analyses. In this chapter, we introduce the protocol for the nontargeted gas chromatography/mass spectrometry (GC/MS) based metabolomics.",

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T1 - Gas Chromatography/Mass Spectrometry Analysis: Nontargeted Metabolomics Based on Scan Mode Analysis

AU - Tsugawa, H.

AU - Lommen, A.

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N2 - Metabolome analysis is divided into two approaches: nontargeted analysis and targeted analysis.1 Nontargeted analysis covers all detected peaks by means of scan mode and utilizes both annotated and unannotated peak information for statistical analyses. This approach has been broadly applied to discovery-phase studies such as biomarker identification for medical diagnostics. On the other hand, the targeted or widely targeted analysis hunts only “known metabolites” detected by single ion monitoring or selected reaction monitoring mode with the purpose of validating a biological hypothesis or machine-learning studies such as regression and discriminant analyses. In this chapter, we introduce the protocol for the nontargeted gas chromatography/mass spectrometry (GC/MS) based metabolomics.

AB - Metabolome analysis is divided into two approaches: nontargeted analysis and targeted analysis.1 Nontargeted analysis covers all detected peaks by means of scan mode and utilizes both annotated and unannotated peak information for statistical analyses. This approach has been broadly applied to discovery-phase studies such as biomarker identification for medical diagnostics. On the other hand, the targeted or widely targeted analysis hunts only “known metabolites” detected by single ion monitoring or selected reaction monitoring mode with the purpose of validating a biological hypothesis or machine-learning studies such as regression and discriminant analyses. In this chapter, we introduce the protocol for the nontargeted gas chromatography/mass spectrometry (GC/MS) based metabolomics.

U2 - 10.1201/b17793-13

DO - 10.1201/b17793-13

M3 - Chapter

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SN - 9781032652245

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EP - 136

BT - Mass Spectrometry-Based Metabolomics

A2 - Prama Putri, S.

A2 - Fukusaki, E.

PB - CRC Press

CY - Boca Raton

ER -

Gas Chromatography/Mass Spectrometry Analysis: Nontargeted Metabolomics Based on Scan Mode Analysis

Abstract

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Projects

Foodomics metabole profilering (KB-15-001-020)

Cite this