Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

Ruth Schmidt, Victor de Jager, Daniela Zühlke, Christian Wolff, Jörg Bernhardt, Katarina Cankar, Jules Beekwilder, Wilfred van IJcken, Frank Sleutels, Wietse de Boer, Katharina Riedel, Paolina Garbeva

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria.
LanguageEnglish
Article number862
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017

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secondary metabolite
volatile organic compound
bacterium
terpene
fungus
communication
proteomics
gene
motility
pathogen
metabolism
protein
energy

Cite this

Schmidt, Ruth ; de Jager, Victor ; Zühlke, Daniela ; Wolff, Christian ; Bernhardt, Jörg ; Cankar, Katarina ; Beekwilder, Jules ; van IJcken, Wilfred ; Sleutels, Frank ; de Boer, Wietse ; Riedel, Katharina ; Garbeva, Paolina. / Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C. In: Scientific Reports. 2017 ; Vol. 7.
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abstract = "The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria.",
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Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C. / Schmidt, Ruth; de Jager, Victor; Zühlke, Daniela; Wolff, Christian; Bernhardt, Jörg; Cankar, Katarina; Beekwilder, Jules; van IJcken, Wilfred; Sleutels, Frank; de Boer, Wietse; Riedel, Katharina; Garbeva, Paolina.

In: Scientific Reports, Vol. 7, 862, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

AU - Schmidt, Ruth

AU - de Jager, Victor

AU - Zühlke, Daniela

AU - Wolff, Christian

AU - Bernhardt, Jörg

AU - Cankar, Katarina

AU - Beekwilder, Jules

AU - van IJcken, Wilfred

AU - Sleutels, Frank

AU - de Boer, Wietse

AU - Riedel, Katharina

AU - Garbeva, Paolina

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AB - The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria.

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