Abstract
Dehydroabietic acid (DHA) (1) is one of the main compounds in Scots pine wood responsible for aquatic and microbial toxicity. The degradation of 1 by Trametes versicolor and Phlebiopsis gigantea in liquid stationary cultures was followed by HPLC-DAD-ELSD. Both fungi rapidly degraded DHA relative to a control. More breakdown products were observed for T. versicolor than for P. gigantea. After 13 days, four compounds were identified by means of spectroscopic methods in P. gigantea cultures: 1 beta-hydroxy-DHA (2), 1 beta,7 alpha-dihydroxy-DHA (3), 1 beta,16-dihydroxy-DHA (5), and tentatively 1 beta-hydroxy-7-oxo-DHA (4). In T. versicolor cultures, 1 beta,16-dihydroxy-DHA (5), 7 beta,16-dihydroxy-DHA (6), 1 beta,7 beta,16-trihydroxy-DHA (7), 1 beta,16-dihydroxy-7-oxo-DHA (8), 1 beta,15-dihydroxy-DHA (9), and 1 beta,7 alpha,16-trihydroxy-DHA (10) were identified after 9 days of incubation. Thus the biotransformation of 1 by the two fungi was different, with only 5 being produced by both strains. Compounds 3, 7, 8, and 10 are reported for the first time as natural products.
Original language | English |
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Pages (from-to) | 154-159 |
Journal | Journal of Natural Products |
Volume | 70 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2007 |
Keywords
- pseudomonas-abietaniphila bkme-9
- mill effluent
- kraft pulp
- mortierella-isabellina
- bacterial degradation
- resin acids
- paper-mill
- hydroxylation
- diterpenoids
- constituents