Functional Region Identification in Proteins by Accumulative-Quantitative Peptide Mapping Using RP-HPLC-MS

B.J.H. Kuipers, E.J. Bakx, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

A new method was developed to identify regions in proteins from which peptides are derived with specific functional properties. This method is applicable for systems in which peptides of a hydrolyzed protein possess specific functional properties, but are too large to be sequenced directly and/or the peptide mixture is too complex to purify and characterize each peptide individually. In the present work, aggregating peptides obtained by proteolytic hydrolysis of soy glycinin were used as a case study. The aggregating peptides are isolated and subsequently further degraded with trypsin to result in peptides with a mass
Original languageEnglish
Pages (from-to)9337-9344
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number23
DOIs
Publication statusPublished - 2007

Keywords

  • mass-spectrometry
  • soy proteins
  • absorption
  • allergen
  • acids

Fingerprint Dive into the research topics of 'Functional Region Identification in Proteins by Accumulative-Quantitative Peptide Mapping Using RP-HPLC-MS'. Together they form a unique fingerprint.

  • Cite this