Functional imaging of plants: A nuclear magnetic resonance study of a cucumber plant

T. Scheenen, A. Heemskerk, A. de Jager, F.J. Vergeldt, H. van As

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

Functional magnetic resonance imaging was used to study transients of biophysical parameters in a cucumber plant in response to environmental changes. Detailed flow imaging experiments showed the location of xylem and phloem in the stem and the response of the following flow characteristics to the imposed environmental changes: the total amount of water, the amount of stationary and flowing water, the linear velocity of the flowing water, and the volume flow. The total measured volume flow through the plant stem was in good agreement with the independently measured water uptake by the roots. A separate analysis of the flow characteristics for two vascular bundles revealed that changes in volume flow of the xylem sap were accounted for by a change in linear-flow velocities in the xylem vessels. Multiple-spin echo experiments revealed two water fractions for different tissues in the plant stem; the spin-spin relaxation time of the larger fraction of parenchyma tissue in the center of the stem and the vascular tissue was down by 17% in the period after cooling the roots of the plant. This could point to an increased water permeability of the tonoplast membrane of the observed cells in this period of quick recovery from severe water loss
LanguageEnglish
Pages481-492
JournalBiophysical Journal
Volume82
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Cucumis sativus
Magnetic Resonance Spectroscopy
Water
Xylem
Plant Stems
Blood Vessels
Phloem
Plant Roots
Permeability
Magnetic Resonance Imaging
Cell Membrane

Keywords

  • nmr-microscopy
  • noninvasive measurement
  • water transport
  • sap ascent
  • flow
  • relaxation
  • xylem
  • tissues
  • h-1-nmr
  • phloem

Cite this

Scheenen, T. ; Heemskerk, A. ; de Jager, A. ; Vergeldt, F.J. ; van As, H. / Functional imaging of plants: A nuclear magnetic resonance study of a cucumber plant. In: Biophysical Journal. 2002 ; Vol. 82, No. 1. pp. 481-492.
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abstract = "Functional magnetic resonance imaging was used to study transients of biophysical parameters in a cucumber plant in response to environmental changes. Detailed flow imaging experiments showed the location of xylem and phloem in the stem and the response of the following flow characteristics to the imposed environmental changes: the total amount of water, the amount of stationary and flowing water, the linear velocity of the flowing water, and the volume flow. The total measured volume flow through the plant stem was in good agreement with the independently measured water uptake by the roots. A separate analysis of the flow characteristics for two vascular bundles revealed that changes in volume flow of the xylem sap were accounted for by a change in linear-flow velocities in the xylem vessels. Multiple-spin echo experiments revealed two water fractions for different tissues in the plant stem; the spin-spin relaxation time of the larger fraction of parenchyma tissue in the center of the stem and the vascular tissue was down by 17{\%} in the period after cooling the roots of the plant. This could point to an increased water permeability of the tonoplast membrane of the observed cells in this period of quick recovery from severe water loss",
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Functional imaging of plants: A nuclear magnetic resonance study of a cucumber plant. / Scheenen, T.; Heemskerk, A.; de Jager, A.; Vergeldt, F.J.; van As, H.

In: Biophysical Journal, Vol. 82, No. 1, 2002, p. 481-492.

Research output: Contribution to journalArticleAcademicpeer-review

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