The mustard trypsin inhibitor MTI-2 is a potential tool in the study of interactions between pest insects and plants. It can be applied to study the adaptations of digestive proteases in pest insects. Phage display allows a rapid and exhaustive system for the selection of heterologous protein variants with novel specificities. Here we describe a bacteriophage expression system which permits functional expression of MTI-2 variants. Active and inactive mutants of MTI-2 are constructed and displayed on phage. These are used to demonstrate that an active variant can be selected from a background of 10,000 inactive mutants in four rounds of selection and amplification.
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2001|
Volpicella, M., Ceci, L. R., Gallerani, R., Jongsma, M. A., & Beekwilder, J. (2001). Functional expression on bacteriophage of the mustard trypsin inhibitor MTI-2. Biochemical and Biophysical Research Communications, 280, 813-817. https://doi.org/10.1006/bbrc.2000.4182