Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.)

Valentina Bracuto, Michela Appiano, Luigi Ricciardi, Deniz Göl, Richard G.F. Visser, Yuling Bai*, Stefano Pavan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Eggplant (Solanum melongena L.) is one of the most important vegetables among the Solanaceae and can be a host to fungal species causing powdery mildew (PM) disease. Specific homologs of the plant Mildew Locus O (MLO) gene family are PM susceptibility factors, as their loss of function results in a recessive form of resistance known as mlo resistance. In a previous work, we isolated the eggplant MLO homolog SmMLO1. SmMLO1 is closely related to MLO susceptibility genes characterized in other plant species. However, it displays a peculiar non-synonymous substitution that leads to a T → M amino acid change at protein position 422, in correspondence of the MLO calmodulin-binding domain. In this study, we performed the functional characterization of SmMLO1. Transgenic overexpression of SmMLO1 in a tomato mlo mutant compromised resistance to the tomato PM pathogen Oidium neolycopersici, thus indicating that SmMLO1 is a PM susceptibility factor in eggplant. PM susceptibility was also restored by the transgenic expression of a synthetic gene, named s-SmMLO1, encoding a protein identical to SmMLO1, except for the presence of T at position 422. This indicates that the T → M polymorphism does not affect the protein role as PM susceptibility factor. Overall, the results of this work are of interest for the functional characterization of MLO proteins and the introduction of PM resistance in eggplant using reverse genetics.
Original languageEnglish
Pages (from-to)323-330
JournalTransgenic Research
Volume26
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

Solanum melongena
eggplants
powdery mildew
Genes
loci
Lycopersicon esculentum
genes
Proteins
Solanaceae
Synthetic Genes
Reverse Genetics
Oidium neolycopersici
proteins
Calmodulin
tomatoes
Vegetables
synthetic genes
calmodulin
Amino Acids
vegetables

Keywords

  • Eggplant
  • MLO
  • Plant breeding
  • Powdery mildew
  • Resistance

Cite this

Bracuto, Valentina ; Appiano, Michela ; Ricciardi, Luigi ; Göl, Deniz ; Visser, Richard G.F. ; Bai, Yuling ; Pavan, Stefano. / Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.). In: Transgenic Research. 2017 ; Vol. 26, No. 3. pp. 323-330.
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abstract = "Eggplant (Solanum melongena L.) is one of the most important vegetables among the Solanaceae and can be a host to fungal species causing powdery mildew (PM) disease. Specific homologs of the plant Mildew Locus O (MLO) gene family are PM susceptibility factors, as their loss of function results in a recessive form of resistance known as mlo resistance. In a previous work, we isolated the eggplant MLO homolog SmMLO1. SmMLO1 is closely related to MLO susceptibility genes characterized in other plant species. However, it displays a peculiar non-synonymous substitution that leads to a T → M amino acid change at protein position 422, in correspondence of the MLO calmodulin-binding domain. In this study, we performed the functional characterization of SmMLO1. Transgenic overexpression of SmMLO1 in a tomato mlo mutant compromised resistance to the tomato PM pathogen Oidium neolycopersici, thus indicating that SmMLO1 is a PM susceptibility factor in eggplant. PM susceptibility was also restored by the transgenic expression of a synthetic gene, named s-SmMLO1, encoding a protein identical to SmMLO1, except for the presence of T at position 422. This indicates that the T → M polymorphism does not affect the protein role as PM susceptibility factor. Overall, the results of this work are of interest for the functional characterization of MLO proteins and the introduction of PM resistance in eggplant using reverse genetics.",
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Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.). / Bracuto, Valentina; Appiano, Michela; Ricciardi, Luigi; Göl, Deniz; Visser, Richard G.F.; Bai, Yuling; Pavan, Stefano.

In: Transgenic Research, Vol. 26, No. 3, 2017, p. 323-330.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.)

AU - Bracuto, Valentina

AU - Appiano, Michela

AU - Ricciardi, Luigi

AU - Göl, Deniz

AU - Visser, Richard G.F.

AU - Bai, Yuling

AU - Pavan, Stefano

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AB - Eggplant (Solanum melongena L.) is one of the most important vegetables among the Solanaceae and can be a host to fungal species causing powdery mildew (PM) disease. Specific homologs of the plant Mildew Locus O (MLO) gene family are PM susceptibility factors, as their loss of function results in a recessive form of resistance known as mlo resistance. In a previous work, we isolated the eggplant MLO homolog SmMLO1. SmMLO1 is closely related to MLO susceptibility genes characterized in other plant species. However, it displays a peculiar non-synonymous substitution that leads to a T → M amino acid change at protein position 422, in correspondence of the MLO calmodulin-binding domain. In this study, we performed the functional characterization of SmMLO1. Transgenic overexpression of SmMLO1 in a tomato mlo mutant compromised resistance to the tomato PM pathogen Oidium neolycopersici, thus indicating that SmMLO1 is a PM susceptibility factor in eggplant. PM susceptibility was also restored by the transgenic expression of a synthetic gene, named s-SmMLO1, encoding a protein identical to SmMLO1, except for the presence of T at position 422. This indicates that the T → M polymorphism does not affect the protein role as PM susceptibility factor. Overall, the results of this work are of interest for the functional characterization of MLO proteins and the introduction of PM resistance in eggplant using reverse genetics.

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KW - Powdery mildew

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