Functional characterization of Phospholipase D likes in Phytophthora infestans

H.J.G. Meijer, C. Schoina, S. Wang, K. Bouwmeester, C. Hua, F. Govers

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Invasion of plant host tissue by oomycete pathogens, like Phytophthora infestans, is dependent on modifications of the host plasma membrane to facilitate two-way transfer of proteins and other compounds. This modulation of the host plasma membrane, e.g. to establish extrahaustorial membranes, might be accompanied by extracellular (phospho)lipase activities. Phospholipases have been implicated as virulence factors in pathogens and since the P. infestans genome harbors a range of small phospholipase Ds (PLD-likes) with a signal peptide, these oomycetes could use similar weaponry during the infection process. We show that three small PLD-likes, belonging to three PLD classes, are expressed during various oomycete life-stages, including infection. In planta expression of small PLD-likes promotes local cell-death which highly depends on silencing suppression, calcium presence and intact catalytic motifs. We further demonstrate that in planta expression of the wild type small PLD-likes facilitates P. infestans infection and supports sporangia formation. These results suggest that the small PLD-likes are catalytically active and function by executing membrane modifications to support Phytophthora growth and development in planta.
LanguageEnglish
Title of host publicationAbstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017
PublisherGenetics Society of America
Pages240-240
Publication statusPublished - 2 May 2017
Event29th Fungal Genetics Conference - Asilomar Conference Center, Pacific Grove, CA, United States
Duration: 14 Mar 201719 Mar 2017
http://www.genetics-gsa.org/fungal/2017/Abstract%20Book%202017%208x10.pdf

Conference

Conference29th Fungal Genetics Conference
CountryUnited States
CityPacific Grove, CA
Period14/03/1719/03/17
Internet address

Fingerprint

phospholipase D
Oomycetes
Phytophthora infestans
Plantae
plasma membrane
infection
pathogens
sporangia
Phytophthora
signal peptide
cell death
growth and development
virulence
host plants
calcium
genome
proteins
phospholipases

Cite this

Meijer, H. J. G., Schoina, C., Wang, S., Bouwmeester, K., Hua, C., & Govers, F. (2017). Functional characterization of Phospholipase D likes in Phytophthora infestans. In Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017 (pp. 240-240). Genetics Society of America.
Meijer, H.J.G. ; Schoina, C. ; Wang, S. ; Bouwmeester, K. ; Hua, C. ; Govers, F. / Functional characterization of Phospholipase D likes in Phytophthora infestans. Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017. Genetics Society of America, 2017. pp. 240-240
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abstract = "Invasion of plant host tissue by oomycete pathogens, like Phytophthora infestans, is dependent on modifications of the host plasma membrane to facilitate two-way transfer of proteins and other compounds. This modulation of the host plasma membrane, e.g. to establish extrahaustorial membranes, might be accompanied by extracellular (phospho)lipase activities. Phospholipases have been implicated as virulence factors in pathogens and since the P. infestans genome harbors a range of small phospholipase Ds (PLD-likes) with a signal peptide, these oomycetes could use similar weaponry during the infection process. We show that three small PLD-likes, belonging to three PLD classes, are expressed during various oomycete life-stages, including infection. In planta expression of small PLD-likes promotes local cell-death which highly depends on silencing suppression, calcium presence and intact catalytic motifs. We further demonstrate that in planta expression of the wild type small PLD-likes facilitates P. infestans infection and supports sporangia formation. These results suggest that the small PLD-likes are catalytically active and function by executing membrane modifications to support Phytophthora growth and development in planta.",
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Meijer, HJG, Schoina, C, Wang, S, Bouwmeester, K, Hua, C & Govers, F 2017, Functional characterization of Phospholipase D likes in Phytophthora infestans. in Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017. Genetics Society of America, pp. 240-240, 29th Fungal Genetics Conference, Pacific Grove, CA, United States, 14/03/17.

Functional characterization of Phospholipase D likes in Phytophthora infestans. / Meijer, H.J.G.; Schoina, C.; Wang, S.; Bouwmeester, K.; Hua, C.; Govers, F.

Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017. Genetics Society of America, 2017. p. 240-240.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Functional characterization of Phospholipase D likes in Phytophthora infestans

AU - Meijer, H.J.G.

AU - Schoina, C.

AU - Wang, S.

AU - Bouwmeester, K.

AU - Hua, C.

AU - Govers, F.

PY - 2017/5/2

Y1 - 2017/5/2

N2 - Invasion of plant host tissue by oomycete pathogens, like Phytophthora infestans, is dependent on modifications of the host plasma membrane to facilitate two-way transfer of proteins and other compounds. This modulation of the host plasma membrane, e.g. to establish extrahaustorial membranes, might be accompanied by extracellular (phospho)lipase activities. Phospholipases have been implicated as virulence factors in pathogens and since the P. infestans genome harbors a range of small phospholipase Ds (PLD-likes) with a signal peptide, these oomycetes could use similar weaponry during the infection process. We show that three small PLD-likes, belonging to three PLD classes, are expressed during various oomycete life-stages, including infection. In planta expression of small PLD-likes promotes local cell-death which highly depends on silencing suppression, calcium presence and intact catalytic motifs. We further demonstrate that in planta expression of the wild type small PLD-likes facilitates P. infestans infection and supports sporangia formation. These results suggest that the small PLD-likes are catalytically active and function by executing membrane modifications to support Phytophthora growth and development in planta.

AB - Invasion of plant host tissue by oomycete pathogens, like Phytophthora infestans, is dependent on modifications of the host plasma membrane to facilitate two-way transfer of proteins and other compounds. This modulation of the host plasma membrane, e.g. to establish extrahaustorial membranes, might be accompanied by extracellular (phospho)lipase activities. Phospholipases have been implicated as virulence factors in pathogens and since the P. infestans genome harbors a range of small phospholipase Ds (PLD-likes) with a signal peptide, these oomycetes could use similar weaponry during the infection process. We show that three small PLD-likes, belonging to three PLD classes, are expressed during various oomycete life-stages, including infection. In planta expression of small PLD-likes promotes local cell-death which highly depends on silencing suppression, calcium presence and intact catalytic motifs. We further demonstrate that in planta expression of the wild type small PLD-likes facilitates P. infestans infection and supports sporangia formation. These results suggest that the small PLD-likes are catalytically active and function by executing membrane modifications to support Phytophthora growth and development in planta.

M3 - Abstract

SP - 240

EP - 240

BT - Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017

PB - Genetics Society of America

ER -

Meijer HJG, Schoina C, Wang S, Bouwmeester K, Hua C, Govers F. Functional characterization of Phospholipase D likes in Phytophthora infestans. In Abstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017. Genetics Society of America. 2017. p. 240-240