Functional characterization of Phospholipase D likes in Phytophthora infestans

H.J.G. Meijer, C. Schoina, S. Wang, K. Bouwmeester, C. Hua, F. Govers

Research output: Chapter in Book/Report/Conference proceedingAbstract


Invasion of plant host tissue by oomycete pathogens, like Phytophthora infestans, is dependent on modifications of the host plasma membrane to facilitate two-way transfer of proteins and other compounds. This modulation of the host plasma membrane, e.g. to establish extrahaustorial membranes, might be accompanied by extracellular (phospho)lipase activities. Phospholipases have been implicated as virulence factors in pathogens and since the P. infestans genome harbors a range of small phospholipase Ds (PLD-likes) with a signal peptide, these oomycetes could use similar weaponry during the infection process. We show that three small PLD-likes, belonging to three PLD classes, are expressed during various oomycete life-stages, including infection. In planta expression of small PLD-likes promotes local cell-death which highly depends on silencing suppression, calcium presence and intact catalytic motifs. We further demonstrate that in planta expression of the wild type small PLD-likes facilitates P. infestans infection and supports sporangia formation. These results suggest that the small PLD-likes are catalytically active and function by executing membrane modifications to support Phytophthora growth and development in planta.
Original languageEnglish
Title of host publicationAbstract Book 29th Fungal Genetics Conference Asilomar 17, Pacific Grove, CA, USA 14-19 March 2017
PublisherGenetics Society of America
Publication statusPublished - 2 May 2017
Event29th Fungal Genetics Conference - Asilomar Conference Center, Pacific Grove, CA, United States
Duration: 14 Mar 201719 Mar 2017


Conference29th Fungal Genetics Conference
Country/TerritoryUnited States
CityPacific Grove, CA
Internet address


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