Functional characterization of a syntaxin involved in tomato (Solanum lycopersicum) resistance against powdery mildew

Valentina Bracuto, Michela Appiano, Zheng Zheng, Anne-Marie A. Wolters, Zhe Yan, Luigi Ricciardi, Richard G.F. Visser, Stefano Pavan, Yuling Bai*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Specific syntaxins, such as Arabidopsis AtPEN1 and its barley ortholog ROR2, play a major role in plant defense against powdery mildews. Indeed, the impairment of these genes results in increased fungal penetration in both host and non-host interactions. In this study, a genome-wide survey allowed the identification of 21 tomato syntaxins. Two of them, named SlPEN1a and SlPEN1b, are closely related to AtPEN1. RNAi-based silencing of SlPEN1a in a tomato line carrying a loss-of-function mutation of the susceptibility gene SlMLO1 led to compromised resistance toward the tomato powdery mildew fungus Oidium neolycopersici. Moreover, it resulted in a significant increase in the penetration rate of the non-adapted powdery mildew fungus Blumeria graminis f. sp. hordei. Codon-based evolutionary analysis and multiple alignments allowed the detection of amino acid residues that are under purifying selection and are specifically conserved in syntaxins involved in plant-powdery mildew interactions. Our findings provide both insights on the evolution of syntaxins and information about their function which is of interest for future studies on plant–pathogen interactions and tomato breeding.

Original languageEnglish
Article number1573
Number of pages10
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - 20 Sep 2017

Fingerprint

Solanum lycopersicum
powdery mildew
tomatoes
Oidium neolycopersici
mildews
Blumeria graminis f. sp. hordei
fungi
codons
genes
barley
Arabidopsis
amino acids
genome
breeding

Keywords

  • Blumeria graminis f. sp. hordei
  • Mlo resistance
  • Non-host resistance
  • Oidium neolycopersici
  • Tomato syntaxins

Cite this

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title = "Functional characterization of a syntaxin involved in tomato (Solanum lycopersicum) resistance against powdery mildew",
abstract = "Specific syntaxins, such as Arabidopsis AtPEN1 and its barley ortholog ROR2, play a major role in plant defense against powdery mildews. Indeed, the impairment of these genes results in increased fungal penetration in both host and non-host interactions. In this study, a genome-wide survey allowed the identification of 21 tomato syntaxins. Two of them, named SlPEN1a and SlPEN1b, are closely related to AtPEN1. RNAi-based silencing of SlPEN1a in a tomato line carrying a loss-of-function mutation of the susceptibility gene SlMLO1 led to compromised resistance toward the tomato powdery mildew fungus Oidium neolycopersici. Moreover, it resulted in a significant increase in the penetration rate of the non-adapted powdery mildew fungus Blumeria graminis f. sp. hordei. Codon-based evolutionary analysis and multiple alignments allowed the detection of amino acid residues that are under purifying selection and are specifically conserved in syntaxins involved in plant-powdery mildew interactions. Our findings provide both insights on the evolution of syntaxins and information about their function which is of interest for future studies on plant–pathogen interactions and tomato breeding.",
keywords = "Blumeria graminis f. sp. hordei, Mlo resistance, Non-host resistance, Oidium neolycopersici, Tomato syntaxins",
author = "Valentina Bracuto and Michela Appiano and Zheng Zheng and Wolters, {Anne-Marie A.} and Zhe Yan and Luigi Ricciardi and Visser, {Richard G.F.} and Stefano Pavan and Yuling Bai",
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Functional characterization of a syntaxin involved in tomato (Solanum lycopersicum) resistance against powdery mildew. / Bracuto, Valentina; Appiano, Michela; Zheng, Zheng; Wolters, Anne-Marie A.; Yan, Zhe; Ricciardi, Luigi; Visser, Richard G.F.; Pavan, Stefano; Bai, Yuling.

In: Frontiers in Plant Science, Vol. 8, 1573, 20.09.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Functional characterization of a syntaxin involved in tomato (Solanum lycopersicum) resistance against powdery mildew

AU - Bracuto, Valentina

AU - Appiano, Michela

AU - Zheng, Zheng

AU - Wolters, Anne-Marie A.

AU - Yan, Zhe

AU - Ricciardi, Luigi

AU - Visser, Richard G.F.

AU - Pavan, Stefano

AU - Bai, Yuling

PY - 2017/9/20

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N2 - Specific syntaxins, such as Arabidopsis AtPEN1 and its barley ortholog ROR2, play a major role in plant defense against powdery mildews. Indeed, the impairment of these genes results in increased fungal penetration in both host and non-host interactions. In this study, a genome-wide survey allowed the identification of 21 tomato syntaxins. Two of them, named SlPEN1a and SlPEN1b, are closely related to AtPEN1. RNAi-based silencing of SlPEN1a in a tomato line carrying a loss-of-function mutation of the susceptibility gene SlMLO1 led to compromised resistance toward the tomato powdery mildew fungus Oidium neolycopersici. Moreover, it resulted in a significant increase in the penetration rate of the non-adapted powdery mildew fungus Blumeria graminis f. sp. hordei. Codon-based evolutionary analysis and multiple alignments allowed the detection of amino acid residues that are under purifying selection and are specifically conserved in syntaxins involved in plant-powdery mildew interactions. Our findings provide both insights on the evolution of syntaxins and information about their function which is of interest for future studies on plant–pathogen interactions and tomato breeding.

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KW - Blumeria graminis f. sp. hordei

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KW - Non-host resistance

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