Functional analysis of the Verticillium resistance gene Ve1

Z. Zhang

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

PO-54 Functional analysis of the Verticillium resistance gene Ve1 Zhao Zhang Lab of Phytopathology, Wageningen University, the Netherlands Race-specific pathogen resistance in plants is governed by corresponding plant resistance (R) genes and pathogen avirulence (Avr) genes. R protein-mediated recognition of an Avr protein usually activates an hypersensitive response (HR), which is necrosis of plant tissue surrounding the infection site. Verticillium spp. are economically important pathogens that cause vascular wilt diseases in a wide range of plant species worldwide. A locus responsible for resistance against race 1 strains of Verticillium has been cloned from tomato (Solanum lycopersicum). This locus, named Ve, comprises two closely linked inversely oriented R genes, Ve1 and Ve2, which encode extracellular leucine-rich repeat (eLRR) receptor-like proteins (RLPs). It has recently been demonstrated Ve1, but not Ve2, acts as a functional Verticillium resistance gene in tomato and Arabidopsis. Recently, the Verticillium race 1 elicitor that activates Ve1-mediated resistance, Ave1, was identified. Co-expression of the Ave1 and Ve1 through Agrobacterium tumefaciens-mediated transient expression (agroinfiltration) can successfully induce HR in Nicotiana tabacum (tobacco). In this PhD project, several research lines using Arabidopsis and tomato will be followed with the aim to investigate: 1.) localization of Ve1 protein; 2.) Why Ve2 is not a functional resistance protein; 3.) how eLRRs of Ve1 mediate interactions with their ligand Ave1; 4.) what partner proteins Ve1 associates with and the composition of the Ve-mediated defence signal transduction pathway.
LanguageEnglish
Title of host publicationBook of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011
Place of PublicationWageningen, the Netherlands
Pages49-50
Publication statusPublished - 2011
EventEPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands -
Duration: 1 Nov 20113 Nov 2011

Conference

ConferenceEPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands
Period1/11/113/11/11

Fingerprint

Verticillium
genes
proteins
tomatoes
hypersensitive response
pathogens
agroinfiltration
Arabidopsis
vascular wilt
loci
plant pathology
Solanum lycopersicum
research projects
Agrobacterium radiobacter
Nicotiana tabacum
leucine
plant tissues
signal transduction
necrosis
tobacco

Cite this

Zhang, Z. (2011). Functional analysis of the Verticillium resistance gene Ve1. In Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011 (pp. 49-50). Wageningen, the Netherlands.
Zhang, Z. / Functional analysis of the Verticillium resistance gene Ve1. Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, 2011. pp. 49-50
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Zhang, Z 2011, Functional analysis of the Verticillium resistance gene Ve1. in Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, pp. 49-50, EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1/11/11.

Functional analysis of the Verticillium resistance gene Ve1. / Zhang, Z.

Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, 2011. p. 49-50.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

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AB - PO-54 Functional analysis of the Verticillium resistance gene Ve1 Zhao Zhang Lab of Phytopathology, Wageningen University, the Netherlands Race-specific pathogen resistance in plants is governed by corresponding plant resistance (R) genes and pathogen avirulence (Avr) genes. R protein-mediated recognition of an Avr protein usually activates an hypersensitive response (HR), which is necrosis of plant tissue surrounding the infection site. Verticillium spp. are economically important pathogens that cause vascular wilt diseases in a wide range of plant species worldwide. A locus responsible for resistance against race 1 strains of Verticillium has been cloned from tomato (Solanum lycopersicum). This locus, named Ve, comprises two closely linked inversely oriented R genes, Ve1 and Ve2, which encode extracellular leucine-rich repeat (eLRR) receptor-like proteins (RLPs). It has recently been demonstrated Ve1, but not Ve2, acts as a functional Verticillium resistance gene in tomato and Arabidopsis. Recently, the Verticillium race 1 elicitor that activates Ve1-mediated resistance, Ave1, was identified. Co-expression of the Ave1 and Ve1 through Agrobacterium tumefaciens-mediated transient expression (agroinfiltration) can successfully induce HR in Nicotiana tabacum (tobacco). In this PhD project, several research lines using Arabidopsis and tomato will be followed with the aim to investigate: 1.) localization of Ve1 protein; 2.) Why Ve2 is not a functional resistance protein; 3.) how eLRRs of Ve1 mediate interactions with their ligand Ave1; 4.) what partner proteins Ve1 associates with and the composition of the Ve-mediated defence signal transduction pathway.

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Zhang Z. Functional analysis of the Verticillium resistance gene Ve1. In Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands. 2011. p. 49-50