Functional analysis of the transcriptional activator XlnR from Aspergillus niger

A.A. Hasper, L.M. Trindade, D. van der Veen, A.J.J. van Ooyen, L.H. de Graaff

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57 Citations (Scopus)

Abstract

The transcriptional activator XlnR from Aspergillus niger is a zinc binuclear cluster transcription factor that belongs to the GAL4 superfamily. Several putative structural domains in XInR were predicted using database and protein sequence analysis. Thus far, only the functionality of the N-terminal DNA-binding domain has been determined experimentally. Deletion mutants of the xInR gene were constructed to localize the functional regions of the protein. The results showed that a putative C-terminal coiled-coil region is involved in nuclear import of XInR. After deletion of the C-terminus, including the coiled-coil region, XInR was found in the cytoplasm, while deletion of the C-terminus downstream of the coiled-coil region resulted in nuclear import of XlnR. The latter mutant also showed increased xylanase activity, indicating the presence of a region with an inhibitory function in XInR-controlled transcription. Previous findings had already shown that a mutation in the XlnR C-terminal region resulted in transcription of the structural genes under non-inducing conditions. A regulatory model of XInR is presented in which the C-terminus responds to repressing signals, resulting in an inactive state of the protein.
Original languageEnglish
Pages (from-to)1367-1375
JournalMicrobiology
Volume150
DOIs
Publication statusPublished - 2004

Keywords

  • saccharomyces-cerevisiae
  • utilization pathway
  • importin beta
  • d-xylose
  • sequence
  • protein
  • regulator
  • gene
  • expression
  • nidulans

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