Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae

H.P. van Esse

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

PO-09 Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae H. Peter van Esse Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. Plant diseases cause severe crop losses worldwide with devastating effects on food and feed production. Vascular fungal pathogens are particularly notorious because they lack curative treatments and survive for decades in soil via persistent resting structures. The increased need to reduce the use of harmful pesticides and to develop novel control strategies requires in-depth understanding of the biology of vascular pathogens and the molecular mechanisms underlying pathogenicity and survival. The soil-borne vascular pathogen Verticillium dahliae causes wilt disease on more than 200 plant species, including economically important crops and the model plant Arabidopsis. The proteins secreted by pathogens (secretomes) generally determine the outcome of host-pathogen interactions. Bioinformatic analyses of the recently released V. dahliae genome sequence predict over 780 secreted proteins. When excluding the cell wall-degrading enzymes, 460 secretome genes are identified encoding potential effectors that may potentially govern disease establishment. To identify proteins that modulate host immunity, a screen was developed in which secretome cDNAs would be constitutively expressed in Arabidopsis and analyzed for effects on plant defense (by screening for altered susceptibility towards various pathogens). This way, in an initial pilot screen with 45 single exon candidates, two effectors have been identified that affect host immunity. These two V. dahliae effectors will be subjected to further genetic and biochemical analyses to reveal their mode of action.
LanguageEnglish
Title of host publicationBook of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011
Place of PublicationWageningen, the Netherlands
Pages27-28
Publication statusPublished - 2011
EventEPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands -
Duration: 1 Nov 20113 Nov 2011

Conference

ConferenceEPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands
Period1/11/113/11/11

Fingerprint

vascular wilt
Verticillium dahliae
pathogens
blood vessels
immunity
Arabidopsis
Verticillium
crop losses
host-pathogen relationships
proteins
plant pathology
plant diseases and disorders
bioinformatics
exons
soil
Netherlands
mechanism of action
pesticides
pathogenicity
cell walls

Cite this

van Esse, H. P. (2011). Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae. In Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011 (pp. 27-28). Wageningen, the Netherlands.
van Esse, H.P. / Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae. Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, 2011. pp. 27-28
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van Esse, HP 2011, Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae. in Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, pp. 27-28, EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1/11/11.

Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae. / van Esse, H.P.

Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands, 2011. p. 27-28.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

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van Esse HP. Functional analysis of the secretome of the vascular wilt pathogen Verticillium dahliae. In Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. Wageningen, the Netherlands. 2011. p. 27-28