Functional analysis of potato genes involved in quantitative resistance to Phytophthora infestans

J. Du, Z.D. Tian, J. Liu, V.G.A.A. Vleeshouwers, X.L. Shi, C.H. Xie

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

The most significant threat to potato production worldwide is the late blight disease, which is caused by the oomycete pathogen Phytophthora infestans. Based on previous cDNA microarrays and cDNA-amplified fragment length polymorphism analysis, 63 candidate genes that are expected to contribute to developing a durable resistance to late blight were selected for further functional analysis. We performed virus-induced gene silencing (VIGS) to these candidate genes on both Nicotiana benthamiana and potato, subsequently inoculated detached leaves and assessed the resistance level. Ten genes decreased the resistance to P. infestans after VIGS treatment. Among those, a lipoxygenase (LOX; EC 1.13.11.12) and a suberization-associated anionic peroxidase affected the resistance in both N. benthamiana and potato. Our results identify genes that may play a role in quantitative resistance mechanisms to late blight.
Original languageEnglish
Pages (from-to)957-967
JournalMolecular Biology Reports
Volume40
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Phytophthora infestans
Solanum tuberosum
Gene Silencing
Genes
Oomycetes
Amplified Fragment Length Polymorphism Analysis
Viruses
Lipoxygenase
Oligonucleotide Array Sequence Analysis
Tobacco
Complementary DNA

Keywords

  • systemic acquired-resistance
  • beta-aminobutyric acid
  • late blight
  • disease resistance
  • nicotiana-benthamiana
  • anionic peroxidase
  • durable resistance
  • genome sequence
  • virus
  • plant

Cite this

Du, J. ; Tian, Z.D. ; Liu, J. ; Vleeshouwers, V.G.A.A. ; Shi, X.L. ; Xie, C.H. / Functional analysis of potato genes involved in quantitative resistance to Phytophthora infestans. In: Molecular Biology Reports. 2013 ; Vol. 40, No. 2. pp. 957-967.
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Functional analysis of potato genes involved in quantitative resistance to Phytophthora infestans. / Du, J.; Tian, Z.D.; Liu, J.; Vleeshouwers, V.G.A.A.; Shi, X.L.; Xie, C.H.

In: Molecular Biology Reports, Vol. 40, No. 2, 2013, p. 957-967.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Functional analysis of potato genes involved in quantitative resistance to Phytophthora infestans

AU - Du, J.

AU - Tian, Z.D.

AU - Liu, J.

AU - Vleeshouwers, V.G.A.A.

AU - Shi, X.L.

AU - Xie, C.H.

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AB - The most significant threat to potato production worldwide is the late blight disease, which is caused by the oomycete pathogen Phytophthora infestans. Based on previous cDNA microarrays and cDNA-amplified fragment length polymorphism analysis, 63 candidate genes that are expected to contribute to developing a durable resistance to late blight were selected for further functional analysis. We performed virus-induced gene silencing (VIGS) to these candidate genes on both Nicotiana benthamiana and potato, subsequently inoculated detached leaves and assessed the resistance level. Ten genes decreased the resistance to P. infestans after VIGS treatment. Among those, a lipoxygenase (LOX; EC 1.13.11.12) and a suberization-associated anionic peroxidase affected the resistance in both N. benthamiana and potato. Our results identify genes that may play a role in quantitative resistance mechanisms to late blight.

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KW - disease resistance

KW - nicotiana-benthamiana

KW - anionic peroxidase

KW - durable resistance

KW - genome sequence

KW - virus

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