Functional Analysis of Pathogenicity Proteins of the Potato Cyst Nematode Globodera rostochiensis Using RNAi

C. Qing, S. Rehman, G. Smant, J.T. Jones

Research output: Contribution to journalArticleAcademicpeer-review

121 Citations (Scopus)

Abstract

RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted ß-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the ß-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.
Original languageEnglish
Pages (from-to)621-625
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number7
DOIs
Publication statusPublished - 2005

Keywords

  • double-stranded-rna
  • caenorhabditis-elegans
  • heterodera-glycines
  • expression
  • beta-1,4-endoglucanase
  • gene
  • interference
  • parasitism
  • pallida
  • amphids

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