Fully automated compound screening in Arabidopsis thaliana seedlings

Dominique Audenaert, Long Nguyen, Bert De Rybel, Tom Beeckman

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

High-throughput small molecule screenings in model plants are of great value to identify compounds that interfere with plant developmental processes. In academic research, the plant Arabidopsis thaliana is the most commonly used model organism for this purpose. However, compared to plant cellular systems, A. thaliana plants are less amenable to develop high-throughput screening assays. In this chapter, we describe a screening procedure that is compatible with liquid handling systems and increases the throughput of compound screenings in A. thaliana seedlings.

Original languageEnglish
Title of host publicationPlant Chemical Genomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages3-9
Number of pages7
ISBN (Print)9781627035910
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1056
ISSN (Print)1064-3745

Keywords

  • Arabidopsis thaliana
  • Automation
  • High-throughput screening

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