Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide

Esther Blanco, Beatriz Guerra, Beatriz de la Torre, Sira Defaus, A. Dekker, D. Andreu, Francisco Sobrino

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136–154)] linked through thioether bonds to a T-cell epitope [3A(21–35)] of FMDV [B4T(thi)] elicits potent B- and T-cell specific responses and confers solid protection in pigs to type C FMDV challenge. Herein we show that downsized versions of this peptide bearing two copies of a B-cell epitope from a type O isolate and using thioether [B2T(thi)] or maleimide [B2T(mal)] conjugation chemistries for their synthesis elicited in swine similar or higher B and T-cell specific responses than tetravalent B4T(thi). Moreover, while partial protection was observed in animals immunized with B4T(thi) (60%) and B2T(thi) (80%), B2T(mal) conferred full (100%) protection against FMDV challenge, associated to high levels of circulating IgG2 and mucosal IgGA, and entirely prevented virus shedding. Interestingly, B2T(mal) is also the most advantageous option in terms of synthetic practicality. Taken together, the results reported here point out to B2T(mal) as a highly valuable, cost-effective FMDV candidate vaccine.
Original languageEnglish
Pages (from-to)74-80
JournalAntiviral Research
Volume129
DOIs
Publication statusPublished - 2016

Fingerprint

B-Lymphocyte Epitopes
Foot-and-Mouth Disease
Dendrimers
Foot-and-Mouth Disease Virus
Swine
Peptides
Sulfides
Virus Shedding
T-Lymphocytes
T-Lymphocyte Epitopes
Vaccines
Immunoglobulin G
Costs and Cost Analysis

Cite this

Blanco, Esther ; Guerra, Beatriz ; de la Torre, Beatriz ; Defaus, Sira ; Dekker, A. ; Andreu, D. ; Sobrino, Francisco. / Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide. In: Antiviral Research. 2016 ; Vol. 129. pp. 74-80.
@article{bc639b51ab15416393269ccb7a544c86,
title = "Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide",
abstract = "Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136–154)] linked through thioether bonds to a T-cell epitope [3A(21–35)] of FMDV [B4T(thi)] elicits potent B- and T-cell specific responses and confers solid protection in pigs to type C FMDV challenge. Herein we show that downsized versions of this peptide bearing two copies of a B-cell epitope from a type O isolate and using thioether [B2T(thi)] or maleimide [B2T(mal)] conjugation chemistries for their synthesis elicited in swine similar or higher B and T-cell specific responses than tetravalent B4T(thi). Moreover, while partial protection was observed in animals immunized with B4T(thi) (60{\%}) and B2T(thi) (80{\%}), B2T(mal) conferred full (100{\%}) protection against FMDV challenge, associated to high levels of circulating IgG2 and mucosal IgGA, and entirely prevented virus shedding. Interestingly, B2T(mal) is also the most advantageous option in terms of synthetic practicality. Taken together, the results reported here point out to B2T(mal) as a highly valuable, cost-effective FMDV candidate vaccine.",
author = "Esther Blanco and Beatriz Guerra and {de la Torre}, Beatriz and Sira Defaus and A. Dekker and D. Andreu and Francisco Sobrino",
year = "2016",
doi = "10.1016/j.antiviral.2016.03.005",
language = "English",
volume = "129",
pages = "74--80",
journal = "Antiviral Research",
issn = "0166-3542",
publisher = "Elsevier",

}

Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide. / Blanco, Esther; Guerra, Beatriz; de la Torre, Beatriz; Defaus, Sira; Dekker, A.; Andreu, D.; Sobrino, Francisco.

In: Antiviral Research, Vol. 129, 2016, p. 74-80.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide

AU - Blanco, Esther

AU - Guerra, Beatriz

AU - de la Torre, Beatriz

AU - Defaus, Sira

AU - Dekker, A.

AU - Andreu, D.

AU - Sobrino, Francisco

PY - 2016

Y1 - 2016

N2 - Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136–154)] linked through thioether bonds to a T-cell epitope [3A(21–35)] of FMDV [B4T(thi)] elicits potent B- and T-cell specific responses and confers solid protection in pigs to type C FMDV challenge. Herein we show that downsized versions of this peptide bearing two copies of a B-cell epitope from a type O isolate and using thioether [B2T(thi)] or maleimide [B2T(mal)] conjugation chemistries for their synthesis elicited in swine similar or higher B and T-cell specific responses than tetravalent B4T(thi). Moreover, while partial protection was observed in animals immunized with B4T(thi) (60%) and B2T(thi) (80%), B2T(mal) conferred full (100%) protection against FMDV challenge, associated to high levels of circulating IgG2 and mucosal IgGA, and entirely prevented virus shedding. Interestingly, B2T(mal) is also the most advantageous option in terms of synthetic practicality. Taken together, the results reported here point out to B2T(mal) as a highly valuable, cost-effective FMDV candidate vaccine.

AB - Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136–154)] linked through thioether bonds to a T-cell epitope [3A(21–35)] of FMDV [B4T(thi)] elicits potent B- and T-cell specific responses and confers solid protection in pigs to type C FMDV challenge. Herein we show that downsized versions of this peptide bearing two copies of a B-cell epitope from a type O isolate and using thioether [B2T(thi)] or maleimide [B2T(mal)] conjugation chemistries for their synthesis elicited in swine similar or higher B and T-cell specific responses than tetravalent B4T(thi). Moreover, while partial protection was observed in animals immunized with B4T(thi) (60%) and B2T(thi) (80%), B2T(mal) conferred full (100%) protection against FMDV challenge, associated to high levels of circulating IgG2 and mucosal IgGA, and entirely prevented virus shedding. Interestingly, B2T(mal) is also the most advantageous option in terms of synthetic practicality. Taken together, the results reported here point out to B2T(mal) as a highly valuable, cost-effective FMDV candidate vaccine.

U2 - 10.1016/j.antiviral.2016.03.005

DO - 10.1016/j.antiviral.2016.03.005

M3 - Article

VL - 129

SP - 74

EP - 80

JO - Antiviral Research

JF - Antiviral Research

SN - 0166-3542

ER -