Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity

J.J. de Vega, B. Ramos, B.P.H.J. Thomma, J.M. Díaz-Mínguez

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

The gene ftf2, encoding a transcription factor with a Zn(II)2-Cys6 binuclear cluster DNA- binding motif, was discovered in Fusarium oxysporum f.sp. phaseoli and homologs are found in other pathogenic and non-pathogenic fungi. Southern and genome analyses detected a single ftf2 copy. Previously, a multicopy gene (ftf1) was characterized that is highly homologous to ftf2. Ftf1 is only present in highly virulent strains of F. oxysporum and is dramatically upregulated during the early stages of the infection. To date, however, the function of the ftf2 gene product is still unknown. Silencing of ftf2 was induced in F. oxysporum. Agrobacterium tumefaciens- mediated transfer of a hairpin-expression vector carrying fragments of the ftf2 gene allowed efficient silencing of ftf2 expression. The silenced transformants showed a reduction in their growing capacities and, possibly, a reduction in sporulation. However, no effect on pathogenicity was observed. Also, Real-time PCR analysis indicated that the expression of ftf2 is similar in all the strains and rather constant in time, suggesting a constitutive gene expression pattern for ftf2. Also, no upregulation or downregulations was observed in planta, confirming the absence of an important role for ftf2 in the infection process. Finally, in order to shed some light on the kind of genes regulated by the ftf2 transcription factor, a SSH library was constructed. Two mRNA populations were used that were obtained from the weakly virulent wild type strain FOP-SP4 and a ftf2 silenced FOP-SP4 transformant. Preliminary analysis of the library indicates the presence of genes that are expressed in the tester strain yet absent from the driver RNA, and thus are putatively regulated by the ftf2 transcription factor.
Original languageEnglish
Title of host publicationBook of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009
Pages141 (224)
Publication statusPublished - 2009
Event25th Fungal Genetics Conference, Pacific Grove, California, USA -
Duration: 17 Mar 200922 Mar 2009

Conference

Conference25th Fungal Genetics Conference, Pacific Grove, California, USA
Period17/03/0922/03/09

Fingerprint

pathogenicity
transcription factors
genes
Fusarium oxysporum
Fusarium oxysporum f. sp. phaseoli
DNA-binding domains
Plantae
Agrobacterium radiobacter
sporulation
infection
quantitative polymerase chain reaction
RNA
gene expression
fungi
genome

Cite this

de Vega, J. J., Ramos, B., Thomma, B. P. H. J., & Díaz-Mínguez, J. M. (2009). Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity. In Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009 (pp. 141 (224))
de Vega, J.J. ; Ramos, B. ; Thomma, B.P.H.J. ; Díaz-Mínguez, J.M. / Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity. Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009. 2009. pp. 141 (224)
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abstract = "The gene ftf2, encoding a transcription factor with a Zn(II)2-Cys6 binuclear cluster DNA- binding motif, was discovered in Fusarium oxysporum f.sp. phaseoli and homologs are found in other pathogenic and non-pathogenic fungi. Southern and genome analyses detected a single ftf2 copy. Previously, a multicopy gene (ftf1) was characterized that is highly homologous to ftf2. Ftf1 is only present in highly virulent strains of F. oxysporum and is dramatically upregulated during the early stages of the infection. To date, however, the function of the ftf2 gene product is still unknown. Silencing of ftf2 was induced in F. oxysporum. Agrobacterium tumefaciens- mediated transfer of a hairpin-expression vector carrying fragments of the ftf2 gene allowed efficient silencing of ftf2 expression. The silenced transformants showed a reduction in their growing capacities and, possibly, a reduction in sporulation. However, no effect on pathogenicity was observed. Also, Real-time PCR analysis indicated that the expression of ftf2 is similar in all the strains and rather constant in time, suggesting a constitutive gene expression pattern for ftf2. Also, no upregulation or downregulations was observed in planta, confirming the absence of an important role for ftf2 in the infection process. Finally, in order to shed some light on the kind of genes regulated by the ftf2 transcription factor, a SSH library was constructed. Two mRNA populations were used that were obtained from the weakly virulent wild type strain FOP-SP4 and a ftf2 silenced FOP-SP4 transformant. Preliminary analysis of the library indicates the presence of genes that are expressed in the tester strain yet absent from the driver RNA, and thus are putatively regulated by the ftf2 transcription factor.",
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de Vega, JJ, Ramos, B, Thomma, BPHJ & Díaz-Mínguez, JM 2009, Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity. in Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009. pp. 141 (224), 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17/03/09.

Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity. / de Vega, J.J.; Ramos, B.; Thomma, B.P.H.J.; Díaz-Mínguez, J.M.

Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009. 2009. p. 141 (224).

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity

AU - de Vega, J.J.

AU - Ramos, B.

AU - Thomma, B.P.H.J.

AU - Díaz-Mínguez, J.M.

PY - 2009

Y1 - 2009

N2 - The gene ftf2, encoding a transcription factor with a Zn(II)2-Cys6 binuclear cluster DNA- binding motif, was discovered in Fusarium oxysporum f.sp. phaseoli and homologs are found in other pathogenic and non-pathogenic fungi. Southern and genome analyses detected a single ftf2 copy. Previously, a multicopy gene (ftf1) was characterized that is highly homologous to ftf2. Ftf1 is only present in highly virulent strains of F. oxysporum and is dramatically upregulated during the early stages of the infection. To date, however, the function of the ftf2 gene product is still unknown. Silencing of ftf2 was induced in F. oxysporum. Agrobacterium tumefaciens- mediated transfer of a hairpin-expression vector carrying fragments of the ftf2 gene allowed efficient silencing of ftf2 expression. The silenced transformants showed a reduction in their growing capacities and, possibly, a reduction in sporulation. However, no effect on pathogenicity was observed. Also, Real-time PCR analysis indicated that the expression of ftf2 is similar in all the strains and rather constant in time, suggesting a constitutive gene expression pattern for ftf2. Also, no upregulation or downregulations was observed in planta, confirming the absence of an important role for ftf2 in the infection process. Finally, in order to shed some light on the kind of genes regulated by the ftf2 transcription factor, a SSH library was constructed. Two mRNA populations were used that were obtained from the weakly virulent wild type strain FOP-SP4 and a ftf2 silenced FOP-SP4 transformant. Preliminary analysis of the library indicates the presence of genes that are expressed in the tester strain yet absent from the driver RNA, and thus are putatively regulated by the ftf2 transcription factor.

AB - The gene ftf2, encoding a transcription factor with a Zn(II)2-Cys6 binuclear cluster DNA- binding motif, was discovered in Fusarium oxysporum f.sp. phaseoli and homologs are found in other pathogenic and non-pathogenic fungi. Southern and genome analyses detected a single ftf2 copy. Previously, a multicopy gene (ftf1) was characterized that is highly homologous to ftf2. Ftf1 is only present in highly virulent strains of F. oxysporum and is dramatically upregulated during the early stages of the infection. To date, however, the function of the ftf2 gene product is still unknown. Silencing of ftf2 was induced in F. oxysporum. Agrobacterium tumefaciens- mediated transfer of a hairpin-expression vector carrying fragments of the ftf2 gene allowed efficient silencing of ftf2 expression. The silenced transformants showed a reduction in their growing capacities and, possibly, a reduction in sporulation. However, no effect on pathogenicity was observed. Also, Real-time PCR analysis indicated that the expression of ftf2 is similar in all the strains and rather constant in time, suggesting a constitutive gene expression pattern for ftf2. Also, no upregulation or downregulations was observed in planta, confirming the absence of an important role for ftf2 in the infection process. Finally, in order to shed some light on the kind of genes regulated by the ftf2 transcription factor, a SSH library was constructed. Two mRNA populations were used that were obtained from the weakly virulent wild type strain FOP-SP4 and a ftf2 silenced FOP-SP4 transformant. Preliminary analysis of the library indicates the presence of genes that are expressed in the tester strain yet absent from the driver RNA, and thus are putatively regulated by the ftf2 transcription factor.

M3 - Abstract

SP - 141 (224)

BT - Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009

ER -

de Vega JJ, Ramos B, Thomma BPHJ, Díaz-Mínguez JM. Ftf2 encodes a fungal transcription factor involved in growth but not in pathogenicity. In Book of Abstracts 25th Fungal Genetics Conference, Pacific Grove, California, USA, 17-22 March 2009. 2009. p. 141 (224)