From 13C-lignin to 13C-mycelium: Agaricus bisporus uses polymeric lignin as a carbon source

Katharina Duran, Michael Kohlstedt, Gijs van Erven, Cynthia E. Klostermann, Antoine H.P. America, Edwin Bakx, Johan J.P. Baars, Antonie Gorissen, Ries de Visser, Ronald P. de Vries, Christoph Wittmann, Rob N.J. Comans, Thomas W. Kuyper, Mirjam A. Kabel*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Plant biomass conversion by saprotrophic fungi plays a pivotal role in terrestrial carbon (C) cycling. The general consensus is that fungi metabolize carbohydrates, while lignin is only degraded and mineralized to CO2. Recent research, however, demonstrated fungal conversion of 13C-monoaromatic compounds into proteinogenic amino acids. To unambiguously prove that polymeric lignin is not merely degraded, but also metabolized, carefully isolated 13C-labeled lignin served as substrate for Agaricus bisporus, the world's most consumed mushroom. The fungus formed a dense mycelial network, secreted lignin-active enzymes, depolymerized, and removed lignin. With a lignin carbon use efficiency of 0.14 (g/g) and fungal biomass enrichment in 13C, we demonstrate that A. bisporus assimilated and further metabolized lignin when offered as C-source. Amino acids were high in 13C-enrichment, while fungal-derived carbohydrates, fatty acids, and ergosterol showed traces of 13C. These results hint at lignin conversion via aromatic ring-cleaved intermediates to central metabolites, underlining lignin's metabolic value for fungi.

Original languageEnglish
Pages (from-to)eadl3419
JournalScience Advances
Volume10
Issue number16
DOIs
Publication statusPublished - 19 Apr 2024

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