FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization

E. Zelazny, J.W. Borst, M. Muylaert, H. Batoko, M.A. Hemminga, F. Chaumont

Research output: Contribution to journalArticleAcademicpeer-review

227 Citations (Scopus)

Abstract

Zea mays plasma membrane intrinsic proteins (ZmPIPs) fall into two groups, ZmPIP1s and ZmPIP2s, that exhibit different water channel activities when expressed in Xenopus oocytes. ZmPIP1s are inactive, whereas ZmPIP2s induce a marked increase in the membrane osmotic water permeability coefficient, Pf. We previously showed that, in Xenopus oocytes, ZmPIP1;2 and ZmPIP2;1 interact to increase the cell Pf. Here, we report the localization and interaction of ZmPIP1s and ZmPIP2s in living maize cells. ZmPIPs were fused to monomeric yellow fluorescent protein and/or monomeric cyan fluorescent protein and expressed transiently in maize mesophyll protoplasts. When expressed alone, ZmPIP1 fusion proteins were retained in the endoplasmic reticulum, whereas ZmPIP2s were found in the plasma membrane. Interestingly, when coexpressed with ZmPIP2s, ZmPIP1s were relocalized to the plasma membrane. Using FRET/fluorescence lifetime imaging microscopy, we demonstrated that this relocalization results from interaction between ZmPIP1s and ZmPIP2s. Immunoprecipitation experiments provided additional evidence for the association of ZmPIP1;2 and ZmPIP2;1 in maize roots and suspension cells. These data suggest that PIP1¿PIP2 interaction is required for in planta PIP1 trafficking to the plasma membrane to modulate plasma membrane permeability.
Original languageEnglish
Pages (from-to)12359-12364
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number30
DOIs
Publication statusPublished - 2007

Keywords

  • protein-protein interactions
  • physiological functions
  • water permeability
  • intrinsic proteins
  • angstrom structure
  • structural basis
  • plant-cells
  • arabidopsis
  • expression
  • heterodimerization

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