FRET-FLIM applications in plant systems

C.A. Bücherl, A.N. Bader, A.H. Westphal, S. Laptenok, J.W. Borst

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

A hallmark of cellular processes is the spatio-temporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination with advances in fluorescence microscopy have revolutionised this field of research by providing the genetic tools to investigate the spatio-temporal dynamics of biomolecules in live cells. In particular, fluorescence lifetime imaging microscopy (FLIM) has become an inevitable technique for spatially resolving cellular processes and physical interactions of cellular components in real time based on the detection of Förster resonance energy transfer (FRET). In this review, we provide a theoretical background of FLIM as well as FRET-FLIM analysis. Furthermore, we show two cases in which advanced microscopy applications revealed many new insights of cellular processes in living plant cells as well as in whole plants.
Original languageEnglish
Pages (from-to)383-394
JournalProtoplasma
Volume251
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • lifetime imaging microscopy
  • resonance energy-transfer
  • fluorescence correlation spectroscopy
  • protein-protein interactions
  • living cells
  • subcellular resolution
  • molecular-interactions
  • spatial-resolution
  • fusion-proteins
  • global analysis

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