Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem

Jos R. Wendrich, Barbara K. Möller, Song Li, Shunsuke Saiga, Rosangela Sozzani, Philip N. Benfey, Bert De Rybel, Dolf Weijers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

In plants, apical meristems allow continuous growth along the body axis. Within the root apical meristem, a group of slowly dividing quiescent center cells is thought to limit stem cell activity to directly neighboring cells, thus endowing them with unique properties, distinct from displaced daughters. This binary identity of the stem cells stands in apparent contradiction to the more gradual changes in cell division potential and differentiation that occur as cells move further away from the quiescent center. To address this paradox and to infer molecular organization of the root meristem, we used a whole-genome approach to determine dominant transcriptional patterns along root ontogeny zones. We found that the prevalent patterns are expressed in two opposing gradients. One is characterized by genes associated with development, the other enriched in differentiation genes. We confirmed these transcript gradients, and demonstrate that these translate to gradients in protein accumulation and gradual changes in cellular properties. We also show that gradients are genetically controlled through multiple pathways. Based on these findings, we propose that cells in the Arabidopsis root meristem gradually transition from stem cell activity toward differentiation.
Original languageEnglish
Pages (from-to)E8922-E8929
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number42
DOIs
Publication statusPublished - 2017

Keywords

  • Arabidopsis
  • Plant development
  • Root meristem
  • Transcriptional regulation

Fingerprint

Dive into the research topics of 'Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem'. Together they form a unique fingerprint.

Cite this