Fractionation of proteins and carbohydrates from crude microalgae extracts using an ionic liquid based-aqueous two phase system

Edgar Suarez Garcia*, Catalina A. Suarez Ruiz, Tewodros Tilaye, Michel H.M. Eppink, Rene H. Wijffels, Corjan van den Berg

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Mild, simple and efficient recovery methods are required to obtain high-value microalgae proteins. As a promising extraction method, an Aqueous two phase system (ATPS) was used to partition proteins from crude microalgae extracts obtained from two green microalgae of industrial interest: Neochloris oleoabundans and Tetraselmis suecica. Furthermore, the Non-Random Two Liquids model (NRTL) was applied to describe both the phase diagram and the partition coefficient of total protein. It was observed that total protein preferentially concentrates in the top phase. Additionally, no significant effect on partition or extraction efficiency was noted at different tie lines. Experimental data indicate that proteins and sugars are selectively fractionated in top and bottom phases respectively. The model provided a good representation of the experimental data for the liquid-liquid equilibrium. Moreover, the model also led to a good representation of the partitioning data for two reference proteins, Rubisco and Bovine Serum Albumin (BSA), as well as for total protein from crude microalgae extracts.

Original languageEnglish
Pages (from-to)56-65
Number of pages10
JournalSeparation and Purification Technology
Volume204
DOIs
Publication statusPublished - 2 Oct 2018

Keywords

  • Crude protein
  • Ionic liquid
  • Partitioning
  • Phase diagram
  • Tie lines

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