Formation of quasi-regular compact structures of poly(methacrylic acid) upon interaction with a-chymotrypsin

E.V. Kudryashova, A.K. Gladilin, V.A. Izumrudov, A. van Hoek, A.J.W.G. Visser, A.V. Levashov

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12 Citations (Scopus)

Abstract

Structure and dynamic properties of free poly(methacrylic acid) (PMA) and PMA complexed with -chymotrypsin (CT) were studied using the time resolved fluorescence anisotropy technique. We have found that the interaction of PMA with CT induces the formation of a quasi-regular structure of PMA. At a CT/PMA weight ratio of 4:1 the interaction with CT leads to formation of approximately four equal segments of polyelectrolyte, each binding one CT molecule and characterized by an independent rotational mobility. Increase of the CT/PMA weight ratio above 8:1 gives rise to the overall rotation of the whole enzyme–polyelectrolyte complex. In water–ethanol mixtures the mobility of PMA segments containing CT decreases and the structure of the complex becomes even more rigid due to enhancement of the electrostatic interaction between CT and PMA. Formation of the compact and quasi-regular structure of the complex is perhaps the main reason behind the enhancement of enzyme stability and suppression of enzyme aggregation in water–organic cosolvent mixtures
Original languageEnglish
Pages (from-to)129-143
JournalBiochimica et biophysica acta-protein structure and molecular enzymology
Volume1550
DOIs
Publication statusPublished - 2001

Keywords

  • α-Chymotrypsin
  • Enzyme-polyelectrolyte complex
  • Pyrene-poly(methacrylic acid)
  • Time resolved fluorescence anisotropy

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