Folding of influenza virus hemagglutinin in insect cells is fast and efficient

X. Li, M.M. van Oers, J.M. Vlak, I. Braakman*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)


Folding of influenza virus hemagglutinin (HA) in the endoplasmic reticulum has been well defined inmammalian cells. In different mammalian cell lines the protein follows the same folding pathway withidentical folding intermediates, but folds with very different kinetics. To examine the effect of cellularcontext on HA folding and to test to which extent insect cells would support the HA folding process,we expressed HA in Sf9 insect cells. Strikingly, in this invertebrate system HA folded faster and moreefficiently, still via the same folding intermediates as in vertebrate cells. Our results suggest that insectcells provide a highly efficient and effective folding environment for influenza virus HA and the idealproduction platform for HA (emergency) vaccines.
Original languageEnglish
Pages (from-to)77-83
JournalJournal of Biotechnology
Publication statusPublished - 2015


  • disulfide bond formation
  • endoplasmic-reticulum
  • quality-control
  • membrane glycoprotein
  • expression system
  • escherichia-coli
  • calnexin
  • vaccine
  • ph
  • oligomerization


Dive into the research topics of 'Folding of influenza virus hemagglutinin in insect cells is fast and efficient'. Together they form a unique fingerprint.

Cite this