Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.
- genetic tools
García-Cayuela, T., de Cadiñanos, L. P., Mohedano, M. L., de Palencia, P. F., Boden, D., Wells, J., ... Requena, T. (2012). Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli. Applied Microbiology and Biotechnology, 96(1), 171-181. https://doi.org/10.1007/s00253-012-4087-z