Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli

T. García-Cayuela; L.P. de Cadiñanos; M.L. Mohedano; P.F. de Palencia; D. Boden; J. Wells; C. Peláez; P. López; T. Requena

doi:10.1007/s00253-012-4087-z

Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli

T. García-Cayuela, L.P. de Cadiñanos, M.L. Mohedano, P.F. de Palencia, D. Boden, J. Wells, C. Peláez, P. López, T. Requena

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.

Original language	English
Pages (from-to)	171-181
Journal	Applied Microbiology and Biotechnology
Volume	96
Issue number	1
DOIs	https://doi.org/10.1007/s00253-012-4087-z
Publication status	Published - 2012

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Keywords

lactococcus-lactis
streptococcus-pneumoniae
genetic tools
in-vitro
green
cremoris
red
transformation
expression
faecalis

Cite this

García-Cayuela, T., de Cadiñanos, L. P., Mohedano, M. L., de Palencia, P. F., Boden, D., Wells, J., ... Requena, T. (2012). Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli. Applied Microbiology and Biotechnology, 96(1), 171-181. https://doi.org/10.1007/s00253-012-4087-z

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abstract = "Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.",

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Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli. / García-Cayuela, T.; de Cadiñanos, L.P.; Mohedano, M.L.; de Palencia, P.F.; Boden, D.; Wells, J.; Peláez, C.; López, P.; Requena, T.

In: Applied Microbiology and Biotechnology, Vol. 96, No. 1, 2012, p. 171-181.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli

AU - García-Cayuela, T.

AU - de Cadiñanos, L.P.

AU - Mohedano, M.L.

AU - de Palencia, P.F.

AU - Boden, D.

AU - Wells, J.

AU - Peláez, C.

AU - López, P.

AU - Requena, T.

PY - 2012

Y1 - 2012

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AB - Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.

KW - lactococcus-lactis

KW - streptococcus-pneumoniae

KW - genetic tools

KW - in-vitro

KW - green

KW - cremoris

KW - red

KW - transformation

KW - expression

KW - faecalis

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DO - 10.1007/s00253-012-4087-z

M3 - Article

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SP - 171

EP - 181

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

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10.1007/s00253-012-4087-z