Projects per year
Abstract
Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.
Original language | English |
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Pages (from-to) | 171-181 |
Journal | Applied Microbiology and Biotechnology |
Volume | 96 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 |
Keywords
- lactococcus-lactis
- streptococcus-pneumoniae
- genetic tools
- in-vitro
- green
- cremoris
- red
- transformation
- expression
- faecalis
Fingerprint Dive into the research topics of 'Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli'. Together they form a unique fingerprint.
Projects
- 1 Finished
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STARS: Scientific Training in Antimicrobial Research Strategies
1/10/09 → 30/09/13
Project: EU research project