Fluorescence quenching of 2-Aminopurine in Dinucleotides

O.J.G. Somsen; A. van Hoek; H. van Amerongen

doi:10.1016/j.cplett.2004.11.122

Fluorescence quenching of 2-Aminopurine in Dinucleotides

O.J.G. Somsen, A. van Hoek, H. van Amerongen

Biophysics

Research output: Contribution to journal › Article › Academic › peer-review

67 Citations (Scopus)

Abstract

2-Aminopurine is a fluorescent base analog that probes local dynamics in DNA because its fluorescence is quenched by the surrounding bases. But, the underlying mechanisms are still under investigation. We have studied fluorescence decay of 2-aminopurine in dinucleotides and found a similar heterogeneity as was previously observed in larger oligonucleotides. This indicates that dinucleotides could function as model for local dynamics of intact DNA. We also found that fast (21–35 ps) quenching could be induced by all natural bases. Our results confirm that the product of this fast transition has the characteristics of a dark state.

Original language	English
Pages (from-to)	61-65
Journal	Chemical Physics Letters
Volume	402
Issue number	1-3
DOIs	https://doi.org/10.1016/j.cplett.2004.11.122
Publication status	Published - 2005

Keywords

time-resolved fluorescence
charge-transfer
electron-transfer
up-conversion
dna duplexes
dynamics
base
nucleotides
spectroscopy
polymerase

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10.1016/j.cplett.2004.11.122

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title = "Fluorescence quenching of 2-Aminopurine in Dinucleotides",

abstract = "2-Aminopurine is a fluorescent base analog that probes local dynamics in DNA because its fluorescence is quenched by the surrounding bases. But, the underlying mechanisms are still under investigation. We have studied fluorescence decay of 2-aminopurine in dinucleotides and found a similar heterogeneity as was previously observed in larger oligonucleotides. This indicates that dinucleotides could function as model for local dynamics of intact DNA. We also found that fast (21–35 ps) quenching could be induced by all natural bases. Our results confirm that the product of this fast transition has the characteristics of a dark state.",

keywords = "time-resolved fluorescence, charge-transfer, electron-transfer, up-conversion, dna duplexes, dynamics, base, nucleotides, spectroscopy, polymerase",

author = "O.J.G. Somsen and {van Hoek}, A. and {van Amerongen}, H.",

year = "2005",

doi = "10.1016/j.cplett.2004.11.122",

language = "English",

volume = "402",

pages = "61--65",

journal = "Chemical Physics Letters",

issn = "0009-2614",

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T1 - Fluorescence quenching of 2-Aminopurine in Dinucleotides

AU - Somsen, O.J.G.

AU - van Hoek, A.

AU - van Amerongen, H.

PY - 2005

Y1 - 2005

N2 - 2-Aminopurine is a fluorescent base analog that probes local dynamics in DNA because its fluorescence is quenched by the surrounding bases. But, the underlying mechanisms are still under investigation. We have studied fluorescence decay of 2-aminopurine in dinucleotides and found a similar heterogeneity as was previously observed in larger oligonucleotides. This indicates that dinucleotides could function as model for local dynamics of intact DNA. We also found that fast (21–35 ps) quenching could be induced by all natural bases. Our results confirm that the product of this fast transition has the characteristics of a dark state.

AB - 2-Aminopurine is a fluorescent base analog that probes local dynamics in DNA because its fluorescence is quenched by the surrounding bases. But, the underlying mechanisms are still under investigation. We have studied fluorescence decay of 2-aminopurine in dinucleotides and found a similar heterogeneity as was previously observed in larger oligonucleotides. This indicates that dinucleotides could function as model for local dynamics of intact DNA. We also found that fast (21–35 ps) quenching could be induced by all natural bases. Our results confirm that the product of this fast transition has the characteristics of a dark state.

KW - time-resolved fluorescence

KW - charge-transfer

KW - electron-transfer

KW - up-conversion

KW - dna duplexes

KW - dynamics

KW - base

KW - nucleotides

KW - spectroscopy

KW - polymerase

U2 - 10.1016/j.cplett.2004.11.122

DO - 10.1016/j.cplett.2004.11.122

M3 - Article

SN - 0009-2614

VL - 402

SP - 61

EP - 65

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 1-3

ER -

Fluorescence quenching of 2-Aminopurine in Dinucleotides

Abstract

Keywords

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