Fluorescence lifetime imaging microscopy of Synechocystis WT cells – Variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803

T. Tóth, V. Chukhutsina, S.K.B. Krumova, Z. Gambos, H. van Amerongen

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

The FLIM (fluorescence lifetime imaging microscopy) technique allows picosecond fluorescence measurements at the level of the individual cell. Using this technique we were able to observe heterogeneity of cyanobacterial cells in a culture grown under controlled conditions and we were able to resolve structural variations within individual cells. It can be concluded that on the one hand the inhomogeneous distribution of photosynthetic pigments within the cell leads to variation of the fluorescence intensity, whereas on the other hand it is impossible to detect variation in the relative amounts of photosystem I and II throughout the cell. Different Synechocystis sp. PCC 6803 strain lines were compared to each other and differences were observed in the average fluorescence lifetimes obtained for individual cells of the various cell lines. The differences can be traced back to variable efficiency of excitation energy transfer from the phycobilisome antenna to the photosystems. We could successfully demonstrate that there is heterogeneity inside individual cells, within individual cultures, and between various wild-type cell lines.
Original languageEnglish
Title of host publicationPhotosynthesis Research for Food, Fuel and Future
Subtitle of host publication15th International Conference on Photosynthesis
EditorsK. Tingyun, L. Congming, Z. Lixin
Place of PublicationBeijing, China
PublisherSpringer
Pages139-142
DOIs
Publication statusPublished - 2012
EventPhotosynthesis Research for Food, Fuel and Future: 15th International Congress on Photosynthesis, Beijing, China -
Duration: 22 Aug 201027 Aug 2010

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Conference

ConferencePhotosynthesis Research for Food, Fuel and Future: 15th International Congress on Photosynthesis, Beijing, China
Period22/08/1027/08/10

Keywords

  • Cyanobacteria
  • Excitation energy transfer
  • FLIM
  • Light harvesting
  • Photosystem

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