Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.

M.A.C.J. Kwaaitaal, M. Schor, M.A. Hink, A.J.W.G. Visser, S.C. de Vries

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

6 Citations (Scopus)

Abstract

Plasma-membrane-localized receptor kinases are essential for cell–cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.
Original languageEnglish
Title of host publicationMethods in Molecular Biology.
Subtitle of host publicationPlant Kinases: Methods and Protocols
EditorsN. Dissmeyer, A. Schnittger
PublisherSpringer
Pages225-242
Volume779
ISBN (Electronic)9781617792649
ISBN (Print)9781617792632
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume779
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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