Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.

M.A.C.J. Kwaaitaal; M. Schor; M.A. Hink; A.J.W.G. Visser; S.C. de Vries

doi:10.1007/978-1-61779-264-9_13

Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.

M.A.C.J. Kwaaitaal, M. Schor, M.A. Hink, A.J.W.G. Visser, S.C. de Vries

Biochemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

7 Citations (Scopus)

Abstract

Plasma-membrane-localized receptor kinases are essential for cell–cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.

Original language	English
Title of host publication	Methods in Molecular Biology.
Subtitle of host publication	Plant Kinases: Methods and Protocols
Editors	N. Dissmeyer, A. Schnittger
Publisher	Springer
Pages	225-242
Volume	779
ISBN (Electronic)	9781617792649
ISBN (Print)	9781617792632
DOIs	https://doi.org/10.1007/978-1-61779-264-9_13
Publication status	Published - 2011

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	779
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Diffusion
FCS
Fluorescence correlation spectroscopy
Fluorescence recovery after photobleaching
FRAP
Mobility
Plasma membrane
Protoplast
Receptor kinase
SERK1

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10.1007/978-1-61779-264-9_13

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Kwaaitaal, M. A. C. J., Schor, M., Hink, M. A., Visser, A. J. W. G., & de Vries, S. C. (2011). Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta. In N. Dissmeyer, & A. Schnittger (Eds.), Methods in Molecular Biology.: Plant Kinases: Methods and Protocols (Vol. 779, pp. 225-242). (Methods in Molecular Biology; Vol. 779). Springer. https://doi.org/10.1007/978-1-61779-264-9_13

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abstract = "Plasma-membrane-localized receptor kinases are essential for cell–cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.",

keywords = "Diffusion, FCS, Fluorescence correlation spectroscopy, Fluorescence recovery after photobleaching, FRAP, Mobility, Plasma membrane, Protoplast, Receptor kinase, SERK1",

author = "M.A.C.J. Kwaaitaal and M. Schor and M.A. Hink and A.J.W.G. Visser and {de Vries}, S.C.",

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Kwaaitaal, MACJ, Schor, M, Hink, MA, Visser, AJWG & de Vries, SC 2011, Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta. in N Dissmeyer & A Schnittger (eds), Methods in Molecular Biology.: Plant Kinases: Methods and Protocols. vol. 779, Methods in Molecular Biology, vol. 779, Springer, pp. 225-242. https://doi.org/10.1007/978-1-61779-264-9_13

Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta. / Kwaaitaal, M.A.C.J.; Schor, M.; Hink, M.A. et al.
Methods in Molecular Biology.: Plant Kinases: Methods and Protocols. ed. / N. Dissmeyer; A. Schnittger. Vol. 779 Springer, 2011. p. 225-242 (Methods in Molecular Biology; Vol. 779).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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T1 - Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.

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AB - Plasma-membrane-localized receptor kinases are essential for cell–cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.

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KW - Protoplast

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Kwaaitaal MACJ, Schor M, Hink MA, Visser AJWG, de Vries SC. Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta. In Dissmeyer N, Schnittger A, editors, Methods in Molecular Biology.: Plant Kinases: Methods and Protocols. Vol. 779. Springer. 2011. p. 225-242. (Methods in Molecular Biology). doi: 10.1007/978-1-61779-264-9_13

Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.

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