FlpS, the FNR-like protein of streptococcus suis is an essential, oxygen-sensing activator of the arginine deiminase system

Jörg Willenborg, Anna Koczula, Marcus Fulde, Astrid de Greeff, Andreas Beineke, Wolfgang Eisenreich, Claudia Huber, Maren Seitz, Peter Valentin-Weigand, Ralph Goethe

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Streptococcus (S.) suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.

LanguageEnglish
Article number51
JournalPathogens
Volume5
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

Streptococcus suis
Oxygen
Proteins
Arginine
Carbon
Protein S
Zoonoses
Gene Expression Profiling
Operon
Infection
Meningitis
Culture Media
Sepsis
Swine
Nucleotides
arginine deiminase
Bacteria
Amino Acids
Survival
Genes

Keywords

  • Arginine deiminase system
  • FNR-like protein
  • Streptococcus suis

Cite this

Willenborg, Jörg ; Koczula, Anna ; Fulde, Marcus ; de Greeff, Astrid ; Beineke, Andreas ; Eisenreich, Wolfgang ; Huber, Claudia ; Seitz, Maren ; Valentin-Weigand, Peter ; Goethe, Ralph. / FlpS, the FNR-like protein of streptococcus suis is an essential, oxygen-sensing activator of the arginine deiminase system. In: Pathogens. 2016 ; Vol. 5, No. 3.
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abstract = "Streptococcus (S.) suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.",
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Willenborg, J, Koczula, A, Fulde, M, de Greeff, A, Beineke, A, Eisenreich, W, Huber, C, Seitz, M, Valentin-Weigand, P & Goethe, R 2016, 'FlpS, the FNR-like protein of streptococcus suis is an essential, oxygen-sensing activator of the arginine deiminase system', Pathogens, vol. 5, no. 3, 51. https://doi.org/10.3390/pathogens5030051

FlpS, the FNR-like protein of streptococcus suis is an essential, oxygen-sensing activator of the arginine deiminase system. / Willenborg, Jörg; Koczula, Anna; Fulde, Marcus; de Greeff, Astrid; Beineke, Andreas; Eisenreich, Wolfgang; Huber, Claudia; Seitz, Maren; Valentin-Weigand, Peter; Goethe, Ralph.

In: Pathogens, Vol. 5, No. 3, 51, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - FlpS, the FNR-like protein of streptococcus suis is an essential, oxygen-sensing activator of the arginine deiminase system

AU - Willenborg, Jörg

AU - Koczula, Anna

AU - Fulde, Marcus

AU - de Greeff, Astrid

AU - Beineke, Andreas

AU - Eisenreich, Wolfgang

AU - Huber, Claudia

AU - Seitz, Maren

AU - Valentin-Weigand, Peter

AU - Goethe, Ralph

PY - 2016

Y1 - 2016

N2 - Streptococcus (S.) suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.

AB - Streptococcus (S.) suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.

KW - Arginine deiminase system

KW - FNR-like protein

KW - Streptococcus suis

U2 - 10.3390/pathogens5030051

DO - 10.3390/pathogens5030051

M3 - Article

VL - 5

JO - Pathogens

T2 - Pathogens

JF - Pathogens

SN - 2076-0817

IS - 3

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ER -