Flowering locus C (FLC) is a potential major regulator of glucosinolate content across developmental stages of Aethionema arabicum (brassicaceae)

Setareh Mohammadin, Phuong Nguyen, Marco S. van Weij, Michael Reichelt, Eric Schranz

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

The biochemical defense of plants can change during their life-cycle and impact herbivore feeding and plant fitness. The annual species Aethionema arabicum is part of the sister clade to all other Brassicaceae. Hence, it holds a phylogenetically important position for studying crucifer trait evolution. Glucosinolates (GS) are essentially Brassicales-specific metabolites involved in plant defense. Using two Ae. arabicum accessions (TUR and CYP) we identify substantial differences in glucosinolate profiles and quantities between lines, tissues and developmental stages. We find tissue specific side-chain modifications in aliphatic GS: methylthioalkyl in leaves, methylsulfinylalkyl in fruits, and methylsulfonylalkyl in seeds. We also find large differences in absolute glucosinolate content between the two accessions (up to 10-fold in fruits) that suggest a regulatory factor is involved that is not part of the quintessential glucosinolate biosynthetic pathway. Consistent with this hypothesis, we identified a single major multi-trait quantitative trait locus controlling total GS concentration across tissues in a recombinant inbred line population derived from TUR and CYP. With fine-mapping, we narrowed the interval to a 58 kb region containing 15 genes, but lacking any known GS biosynthetic genes. The interval contains homologs of both the sulfate transporter SULTR2;1 and FLOWERING LOCUS C. Both loci have diverse functions controlling plant physiological and developmental processes and thus are potential candidates regulating glucosinolate variation across the life-cycle of Aethionema. Future work will investigate changes in gene expression of the candidates genes, the effects of GS variation on insect herbivores and the trade-offs between defense and reproduction.

LanguageEnglish
Article number876
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - 2017

Fingerprint

glucosinolates
Brassicaceae
developmental stages
flowering
loci
life cycle (organisms)
herbivores
Brassicales
fruits
genes
inbred lines
transporters
biochemical pathways
quantitative trait loci
sulfates
metabolites
gene expression
insects
seeds

Keywords

  • Aethionema
  • Brassicaceae
  • Development
  • Glucosinolates
  • Multi-trait analyses
  • QTL

Cite this

@article{9106dd495cc94fe680ab34ed69afce50,
title = "Flowering locus C (FLC) is a potential major regulator of glucosinolate content across developmental stages of Aethionema arabicum (brassicaceae)",
abstract = "The biochemical defense of plants can change during their life-cycle and impact herbivore feeding and plant fitness. The annual species Aethionema arabicum is part of the sister clade to all other Brassicaceae. Hence, it holds a phylogenetically important position for studying crucifer trait evolution. Glucosinolates (GS) are essentially Brassicales-specific metabolites involved in plant defense. Using two Ae. arabicum accessions (TUR and CYP) we identify substantial differences in glucosinolate profiles and quantities between lines, tissues and developmental stages. We find tissue specific side-chain modifications in aliphatic GS: methylthioalkyl in leaves, methylsulfinylalkyl in fruits, and methylsulfonylalkyl in seeds. We also find large differences in absolute glucosinolate content between the two accessions (up to 10-fold in fruits) that suggest a regulatory factor is involved that is not part of the quintessential glucosinolate biosynthetic pathway. Consistent with this hypothesis, we identified a single major multi-trait quantitative trait locus controlling total GS concentration across tissues in a recombinant inbred line population derived from TUR and CYP. With fine-mapping, we narrowed the interval to a 58 kb region containing 15 genes, but lacking any known GS biosynthetic genes. The interval contains homologs of both the sulfate transporter SULTR2;1 and FLOWERING LOCUS C. Both loci have diverse functions controlling plant physiological and developmental processes and thus are potential candidates regulating glucosinolate variation across the life-cycle of Aethionema. Future work will investigate changes in gene expression of the candidates genes, the effects of GS variation on insect herbivores and the trade-offs between defense and reproduction.",
keywords = "Aethionema, Brassicaceae, Development, Glucosinolates, Multi-trait analyses, QTL",
author = "Setareh Mohammadin and Phuong Nguyen and {van Weij}, {Marco S.} and Michael Reichelt and Eric Schranz",
year = "2017",
doi = "10.3389/fpls.2017.00876",
language = "English",
volume = "8",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers",

}

Flowering locus C (FLC) is a potential major regulator of glucosinolate content across developmental stages of Aethionema arabicum (brassicaceae). / Mohammadin, Setareh; Nguyen, Phuong; van Weij, Marco S.; Reichelt, Michael; Schranz, Eric.

In: Frontiers in Plant Science, Vol. 8, 876, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Flowering locus C (FLC) is a potential major regulator of glucosinolate content across developmental stages of Aethionema arabicum (brassicaceae)

AU - Mohammadin, Setareh

AU - Nguyen, Phuong

AU - van Weij, Marco S.

AU - Reichelt, Michael

AU - Schranz, Eric

PY - 2017

Y1 - 2017

N2 - The biochemical defense of plants can change during their life-cycle and impact herbivore feeding and plant fitness. The annual species Aethionema arabicum is part of the sister clade to all other Brassicaceae. Hence, it holds a phylogenetically important position for studying crucifer trait evolution. Glucosinolates (GS) are essentially Brassicales-specific metabolites involved in plant defense. Using two Ae. arabicum accessions (TUR and CYP) we identify substantial differences in glucosinolate profiles and quantities between lines, tissues and developmental stages. We find tissue specific side-chain modifications in aliphatic GS: methylthioalkyl in leaves, methylsulfinylalkyl in fruits, and methylsulfonylalkyl in seeds. We also find large differences in absolute glucosinolate content between the two accessions (up to 10-fold in fruits) that suggest a regulatory factor is involved that is not part of the quintessential glucosinolate biosynthetic pathway. Consistent with this hypothesis, we identified a single major multi-trait quantitative trait locus controlling total GS concentration across tissues in a recombinant inbred line population derived from TUR and CYP. With fine-mapping, we narrowed the interval to a 58 kb region containing 15 genes, but lacking any known GS biosynthetic genes. The interval contains homologs of both the sulfate transporter SULTR2;1 and FLOWERING LOCUS C. Both loci have diverse functions controlling plant physiological and developmental processes and thus are potential candidates regulating glucosinolate variation across the life-cycle of Aethionema. Future work will investigate changes in gene expression of the candidates genes, the effects of GS variation on insect herbivores and the trade-offs between defense and reproduction.

AB - The biochemical defense of plants can change during their life-cycle and impact herbivore feeding and plant fitness. The annual species Aethionema arabicum is part of the sister clade to all other Brassicaceae. Hence, it holds a phylogenetically important position for studying crucifer trait evolution. Glucosinolates (GS) are essentially Brassicales-specific metabolites involved in plant defense. Using two Ae. arabicum accessions (TUR and CYP) we identify substantial differences in glucosinolate profiles and quantities between lines, tissues and developmental stages. We find tissue specific side-chain modifications in aliphatic GS: methylthioalkyl in leaves, methylsulfinylalkyl in fruits, and methylsulfonylalkyl in seeds. We also find large differences in absolute glucosinolate content between the two accessions (up to 10-fold in fruits) that suggest a regulatory factor is involved that is not part of the quintessential glucosinolate biosynthetic pathway. Consistent with this hypothesis, we identified a single major multi-trait quantitative trait locus controlling total GS concentration across tissues in a recombinant inbred line population derived from TUR and CYP. With fine-mapping, we narrowed the interval to a 58 kb region containing 15 genes, but lacking any known GS biosynthetic genes. The interval contains homologs of both the sulfate transporter SULTR2;1 and FLOWERING LOCUS C. Both loci have diverse functions controlling plant physiological and developmental processes and thus are potential candidates regulating glucosinolate variation across the life-cycle of Aethionema. Future work will investigate changes in gene expression of the candidates genes, the effects of GS variation on insect herbivores and the trade-offs between defense and reproduction.

KW - Aethionema

KW - Brassicaceae

KW - Development

KW - Glucosinolates

KW - Multi-trait analyses

KW - QTL

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DO - 10.3389/fpls.2017.00876

M3 - Article

VL - 8

JO - Frontiers in Plant Science

T2 - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 876

ER -