Flooding of the apoplast is a key factor in the development of hyperhydricity

N. van den Dries, S. Gianni, A. Czerednik, F.A. Krens, G.J.M. de Klerk

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

The physiological disorder hyperhydricity occurs frequently in tissue culture and causes several morphological abnormalities such as thick, brittle, curled, and translucent leaves. It is well known that hyperhydric shoots are characterized by a high water content, but how this is related to the abnormalities is not clear. It was observed that water accumulated extensively in the apoplast of leaves of hyperhydric Arabidopsis seedlings and flooded apoplastic air spaces almost completely. In hyperhydric Arabidopsis seedlings, the volume of apoplastic air was reduced from 85% of the apoplast to only 15%. Similar results were obtained with hyperhydric shoots of statice. The elevated expression of hypoxia-responsive genes in hyperhydric seedlings showed that the water saturation of the apoplast decreased oxygen supply. This demonstrates a reduced gas exchange between the symplast and its surroundings, which will consequently lead to the accumulation of gases in the symplast, for example ethylene and methyl jasmonate. The impairment of gas exchange probably brings about the symptoms of hyperhydricity. Interestingly, stomatal aperture was reduced in hyperhydric plants, a previously reported response to injection of water into the apoplast. Closure of the stomata and the accumulation of water in the apoplast may be the reasons why seedlings with a low level of hyperhydricity showed improved acclimatization after planting into soil.
Original languageEnglish
Pages (from-to)5221-5230
JournalJournal of Experimental Botany
Volume64
Issue number16
DOIs
Publication statusPublished - 2013

Fingerprint

hyperhydricity
apoplast
Seedlings
Water
symplast
seedlings
Gases
Arabidopsis
gas exchange
water
Air
air
shoots
Acclimatization
methyl jasmonate
stomata
signs and symptoms (plants)
tissue culture
hypoxia
leaves

Keywords

  • micropropagated carnation shoots
  • in-vitro
  • oxidative stress
  • stomatal closure
  • culture vessels
  • tissue-culture
  • l. shoots
  • vitrification
  • plants
  • leaves

Cite this

van den Dries, N. ; Gianni, S. ; Czerednik, A. ; Krens, F.A. ; de Klerk, G.J.M. / Flooding of the apoplast is a key factor in the development of hyperhydricity. In: Journal of Experimental Botany. 2013 ; Vol. 64, No. 16. pp. 5221-5230.
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abstract = "The physiological disorder hyperhydricity occurs frequently in tissue culture and causes several morphological abnormalities such as thick, brittle, curled, and translucent leaves. It is well known that hyperhydric shoots are characterized by a high water content, but how this is related to the abnormalities is not clear. It was observed that water accumulated extensively in the apoplast of leaves of hyperhydric Arabidopsis seedlings and flooded apoplastic air spaces almost completely. In hyperhydric Arabidopsis seedlings, the volume of apoplastic air was reduced from 85{\%} of the apoplast to only 15{\%}. Similar results were obtained with hyperhydric shoots of statice. The elevated expression of hypoxia-responsive genes in hyperhydric seedlings showed that the water saturation of the apoplast decreased oxygen supply. This demonstrates a reduced gas exchange between the symplast and its surroundings, which will consequently lead to the accumulation of gases in the symplast, for example ethylene and methyl jasmonate. The impairment of gas exchange probably brings about the symptoms of hyperhydricity. Interestingly, stomatal aperture was reduced in hyperhydric plants, a previously reported response to injection of water into the apoplast. Closure of the stomata and the accumulation of water in the apoplast may be the reasons why seedlings with a low level of hyperhydricity showed improved acclimatization after planting into soil.",
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van den Dries, N, Gianni, S, Czerednik, A, Krens, FA & de Klerk, GJM 2013, 'Flooding of the apoplast is a key factor in the development of hyperhydricity', Journal of Experimental Botany, vol. 64, no. 16, pp. 5221-5230. https://doi.org/10.1093/jxb/ert315

Flooding of the apoplast is a key factor in the development of hyperhydricity. / van den Dries, N.; Gianni, S.; Czerednik, A.; Krens, F.A.; de Klerk, G.J.M.

In: Journal of Experimental Botany, Vol. 64, No. 16, 2013, p. 5221-5230.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van den Dries, N.

AU - Gianni, S.

AU - Czerednik, A.

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N2 - The physiological disorder hyperhydricity occurs frequently in tissue culture and causes several morphological abnormalities such as thick, brittle, curled, and translucent leaves. It is well known that hyperhydric shoots are characterized by a high water content, but how this is related to the abnormalities is not clear. It was observed that water accumulated extensively in the apoplast of leaves of hyperhydric Arabidopsis seedlings and flooded apoplastic air spaces almost completely. In hyperhydric Arabidopsis seedlings, the volume of apoplastic air was reduced from 85% of the apoplast to only 15%. Similar results were obtained with hyperhydric shoots of statice. The elevated expression of hypoxia-responsive genes in hyperhydric seedlings showed that the water saturation of the apoplast decreased oxygen supply. This demonstrates a reduced gas exchange between the symplast and its surroundings, which will consequently lead to the accumulation of gases in the symplast, for example ethylene and methyl jasmonate. The impairment of gas exchange probably brings about the symptoms of hyperhydricity. Interestingly, stomatal aperture was reduced in hyperhydric plants, a previously reported response to injection of water into the apoplast. Closure of the stomata and the accumulation of water in the apoplast may be the reasons why seedlings with a low level of hyperhydricity showed improved acclimatization after planting into soil.

AB - The physiological disorder hyperhydricity occurs frequently in tissue culture and causes several morphological abnormalities such as thick, brittle, curled, and translucent leaves. It is well known that hyperhydric shoots are characterized by a high water content, but how this is related to the abnormalities is not clear. It was observed that water accumulated extensively in the apoplast of leaves of hyperhydric Arabidopsis seedlings and flooded apoplastic air spaces almost completely. In hyperhydric Arabidopsis seedlings, the volume of apoplastic air was reduced from 85% of the apoplast to only 15%. Similar results were obtained with hyperhydric shoots of statice. The elevated expression of hypoxia-responsive genes in hyperhydric seedlings showed that the water saturation of the apoplast decreased oxygen supply. This demonstrates a reduced gas exchange between the symplast and its surroundings, which will consequently lead to the accumulation of gases in the symplast, for example ethylene and methyl jasmonate. The impairment of gas exchange probably brings about the symptoms of hyperhydricity. Interestingly, stomatal aperture was reduced in hyperhydric plants, a previously reported response to injection of water into the apoplast. Closure of the stomata and the accumulation of water in the apoplast may be the reasons why seedlings with a low level of hyperhydricity showed improved acclimatization after planting into soil.

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KW - stomatal closure

KW - culture vessels

KW - tissue-culture

KW - l. shoots

KW - vitrification

KW - plants

KW - leaves

U2 - 10.1093/jxb/ert315

DO - 10.1093/jxb/ert315

M3 - Article

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SP - 5221

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JO - Journal of Experimental Botany

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