TY - JOUR
T1 - Flexible gateway constructs for functional analyses of genes in plant pathogenic fungi
AU - Mehrabi, Rahim
AU - Mirzadi Gohari, Amir
AU - da Silva, Gilvan Ferreira
AU - Steinberg, Gero
AU - Kema, Gert H.J.
AU - de Wit, Pierre J.G.M.
PY - 2015
Y1 - 2015
N2 - Genetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nourseothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen.
AB - Genetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nourseothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen.
KW - Binary vectors
KW - Gateway technology
KW - Gene deletion
KW - GFP
KW - RFP
KW - Zymoseptoria tritici
U2 - 10.1016/j.fgb.2015.03.016
DO - 10.1016/j.fgb.2015.03.016
M3 - Article
AN - SCOPUS:84937546952
SN - 1087-1845
VL - 79
SP - 186
EP - 192
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
ER -