TY - JOUR
T1 - Flashing light emitting diodes (LEDs) induce proteins, polyunsaturated fatty acids and pigments in three microalgae
AU - Lima, Serena
AU - Schulze, Peter S.C.
AU - Schüler, Lisa M.
AU - Rautenberger, Ralf
AU - Morales-Sánchez, Daniela
AU - Santos, Tamára F.
AU - Pereira, Hugo
AU - Varela, João C.S.
AU - Scargiali, Francesca
AU - Wijffels, René H.
AU - Kiron, Viswanath
PY - 2021/1/10
Y1 - 2021/1/10
N2 - As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicosapentaenoic acid (EPA) and specific carotenoids up to three-fold compared to continuous or high frequency flashing light (500 Hz, Duty cycle = 0.05). Therefore, applying low-frequency flashing light as finishing step in industrial production can increase protein, polyunsaturated fatty acids or pigment contents in biomass, leading to high-value algal products.
AB - As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicosapentaenoic acid (EPA) and specific carotenoids up to three-fold compared to continuous or high frequency flashing light (500 Hz, Duty cycle = 0.05). Therefore, applying low-frequency flashing light as finishing step in industrial production can increase protein, polyunsaturated fatty acids or pigment contents in biomass, leading to high-value algal products.
KW - Duty cycle
KW - Pigments
KW - PUFA
KW - Pulsed light
KW - Total lipids
U2 - 10.1016/j.jbiotec.2020.11.019
DO - 10.1016/j.jbiotec.2020.11.019
M3 - Article
C2 - 33245925
AN - SCOPUS:85097649129
VL - 325
SP - 15
EP - 24
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
ER -