Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp

H. Marks, J.J.A. Duijse, D. Zuidema, M.C.W. van Hulten, J.M. Vlak

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Abstract

White Spot Syndrome Virus, the type species of the virus family Nimaviridae, is a large dsDNA virus infecting shrimp and other crustaceans. Genomic analysis of three completely sequenced WSSV isolates identified two major polymorphic loci, ¿variable region ORF14/15¿ and ¿variable region ORF23/24¿. Here, we characterize a WSSV isolate originating from shrimp collected in Thailand in 1996 (TH-96-II). This isolate contains the largest WSSV genome (312 kb) identified so far, mainly because of its sequences in both major polymorphic loci. Analysis of ¿variable region ORF14/15¿ suggests that TH-96-II may be ancestral to the WSSV isolates described to date. A comparison for virulence was made between TH-96-II and WSSV-TH, a well characterized isolate containing the smallest genome (293 kb) identified at present. After injection of the isolates into Penaeus monodon the mortality rates showed that the median lethal time (LT50) of TH-96-II was approximately 14 days, compared to 3.5 days for WSSV-TH. When both isolates were mixed in equal amounts and serially passaged in shrimp, WSSV-TH outcompeted TH-96-II within four passages. These data suggest a higher virulence of WSSV-TH compared to TH-96-II. The molecular basis for the difference in virulence remains unclear, but a replication advantage of the 19 kb smaller WSSV-TH genome could play a role
Original languageEnglish
Pages (from-to)9-20
JournalVirus Research
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2005

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White spot syndrome virus 1
Virulence
Genome
Nimaviridae
Penaeidae
Viruses
Thailand
Injections
Mortality

Keywords

  • experimental-infection
  • genome sequence
  • baculovirus
  • wssv
  • thailand
  • wsbv

Cite this

Marks, H. ; Duijse, J.J.A. ; Zuidema, D. ; van Hulten, M.C.W. ; Vlak, J.M. / Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp. In: Virus Research. 2005 ; Vol. 110, No. 1-2. pp. 9-20.
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Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp. / Marks, H.; Duijse, J.J.A.; Zuidema, D.; van Hulten, M.C.W.; Vlak, J.M.

In: Virus Research, Vol. 110, No. 1-2, 2005, p. 9-20.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp

AU - Marks, H.

AU - Duijse, J.J.A.

AU - Zuidema, D.

AU - van Hulten, M.C.W.

AU - Vlak, J.M.

PY - 2005

Y1 - 2005

N2 - White Spot Syndrome Virus, the type species of the virus family Nimaviridae, is a large dsDNA virus infecting shrimp and other crustaceans. Genomic analysis of three completely sequenced WSSV isolates identified two major polymorphic loci, ¿variable region ORF14/15¿ and ¿variable region ORF23/24¿. Here, we characterize a WSSV isolate originating from shrimp collected in Thailand in 1996 (TH-96-II). This isolate contains the largest WSSV genome (312 kb) identified so far, mainly because of its sequences in both major polymorphic loci. Analysis of ¿variable region ORF14/15¿ suggests that TH-96-II may be ancestral to the WSSV isolates described to date. A comparison for virulence was made between TH-96-II and WSSV-TH, a well characterized isolate containing the smallest genome (293 kb) identified at present. After injection of the isolates into Penaeus monodon the mortality rates showed that the median lethal time (LT50) of TH-96-II was approximately 14 days, compared to 3.5 days for WSSV-TH. When both isolates were mixed in equal amounts and serially passaged in shrimp, WSSV-TH outcompeted TH-96-II within four passages. These data suggest a higher virulence of WSSV-TH compared to TH-96-II. The molecular basis for the difference in virulence remains unclear, but a replication advantage of the 19 kb smaller WSSV-TH genome could play a role

AB - White Spot Syndrome Virus, the type species of the virus family Nimaviridae, is a large dsDNA virus infecting shrimp and other crustaceans. Genomic analysis of three completely sequenced WSSV isolates identified two major polymorphic loci, ¿variable region ORF14/15¿ and ¿variable region ORF23/24¿. Here, we characterize a WSSV isolate originating from shrimp collected in Thailand in 1996 (TH-96-II). This isolate contains the largest WSSV genome (312 kb) identified so far, mainly because of its sequences in both major polymorphic loci. Analysis of ¿variable region ORF14/15¿ suggests that TH-96-II may be ancestral to the WSSV isolates described to date. A comparison for virulence was made between TH-96-II and WSSV-TH, a well characterized isolate containing the smallest genome (293 kb) identified at present. After injection of the isolates into Penaeus monodon the mortality rates showed that the median lethal time (LT50) of TH-96-II was approximately 14 days, compared to 3.5 days for WSSV-TH. When both isolates were mixed in equal amounts and serially passaged in shrimp, WSSV-TH outcompeted TH-96-II within four passages. These data suggest a higher virulence of WSSV-TH compared to TH-96-II. The molecular basis for the difference in virulence remains unclear, but a replication advantage of the 19 kb smaller WSSV-TH genome could play a role

KW - experimental-infection

KW - genome sequence

KW - baculovirus

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KW - thailand

KW - wsbv

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