FISH mapping of 57 BAC clones reveals strong conservation of synteny between Galliformes and Anseriformes

V. Fillon, M. Vignoles, R.P.M.A. Crooijmans, M.A.M. Groenen, R. Zoorob, A. Vignal

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

Karyotypes of chicken (Gallus gallus domesticus; 2n = 78) and mallard duck (Anas platyrhynchos; 2n = 80) share the typical organization of avian karyotypes including a few macrochromosome pairs, numerous indistinguishable microchromosomes, and Z and W sex chromosomes. Previous banding studies revealed great similarities between chickens and ducks, but it was not possible to use comparative banding for the microchromosomes. In order to establish precise chromosome correspondences between these two species, particularly for microchromosomes, we hybridized 57 BAC clones previously assigned to the chicken genome to duck metaphase spreads. Although most of the clones showed similar localizations, we found a few intrachromosomal rearrangements of the macrochromosomes and an additional microchromosome pair in ducks. BAC clones specific for chicken microchromosomes were localized to separate duck microchromosomes and clones mapping to the same chicken microchromosome hybridized to the same duck microchromosome, demonstrating a high conservation of synteny. These results demonstrate that the evolution of karyotypes in avian species is the result of fusion and/or fission processes and not translocations.
Original languageEnglish
Pages (from-to)303-307
JournalAnimal Genetics
Volume38
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • chicken gallus-gallus
  • chromosome homology
  • insitu hybridization
  • anas-platyrhynchos
  • japanese-quail
  • evolution
  • genes
  • map
  • microchromosomes
  • domesticus

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