First structural insights into CRISPR-Cas-guided DNA transposition

John van der Oost*, Ioannis Mougiakos

*Corresponding author for this work

Research output: Contribution to journalComment/Letter to the editorAcademic


The transposition mechanism of the Vibrio cholerae Tn6677 transposon (VcTn6677) is based on the unique synergy between a classical transposition machinery (TnsABC-TniQ complex) and a nuclease-deficient type I-F CRISPR-associated complex for antiviral defense (VcCascade). Four independent studies, three of which appeared in Cell Research, recently reported structures of the VcCascade, VcCascade-TniQ, and/or VcCascade-TniQ-dsDNA complexes as well as a TniQ dimer complex, providing the first insights into the assembly and DNA targeting mechanism of a CRISPR-associated transposase complex.
Original languageEnglish
Pages (from-to)193-194
JournalCell Research
Publication statusPublished - 3 Mar 2020

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