First report of southern Tomato virus in tomato in the Canary Islands, Spain

M. Verbeek*, A.M. Dullemans, A. Espino, M. Botella, A. Alfaro-Fernández, M.I. Font

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

In October 2006, tomato plants with torrado disease were sampled in Spain. In a sample of cv. Mariana, originating from Gran Canaria, Tomato torrado virus (ToTV, genus Torradovirus) was detected (isolate GCN06; Alfaro-Fernández et al., 2010). In 2013, the sample was further analysed using next-generation sequencing (NGS). Total RNA, extracted from ca. 150 mg of leaf tissues, was used for an RNA library suitable for Illumina HiSeq paired end sequencing. A de novo assembly (using CLC Genomic Workbench v.6.5) resulted in 1380 sequence contigs. BLAST searches revealed the presence of ToTV and Tomato chlorosis virus (ToCV, genus Crinivirus). One contig (3427 nts, 8901 reads), showed 99.85% identity with Southern tomato virus (STV, genus Amalgavirus), isolate Mexico-1 (GenBank accession No. EF442780), a dsRNA seed-transmitted virus (Sabanadzovic et al., 2009). Reference assembly with the Mexico-1 sequence resulted in a partial sequence of 3420 nts (GenBank KJ174690). The presence of STV in sample GCN06 was verified in RT-PCR using the specific primers STV-fw and STV-rev (Candresse et al., 2013) and sequencing of the obtained amplicon. A new sampling in May 2014 in Gran Canaria revealed the presence of STV in six out of seven tomato samples of cv Mariana. All these samples were co-infected with Pepino mosaic virus (PepMV, genus Potexvirus), Tomato spotted wilt virus (TSWV, genus Tospovirus), and Tomato yellow leaf curl virus (TYLCV, genus Begomovirus). Because STV was generally found in samples co-infected with other viruses, its symptomatology in tomato remains unclear. To our knowledge, this is the first report of STV in the Canary Islands and Spain.
Original languageEnglish
Pages (from-to)392-392
JournalJournal of Plant Pathology: rivista di patologia vegetale
Volume97
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Canary Islands
Spain
tomatoes
viruses
Pepino mosaic virus
Tomato yellow leaf curl virus
sampling
Torradovirus
Tomato torrado virus
Tomato chlorosis virus
Crinivirus
Potexvirus
Mexico
RNA libraries
Tospovirus
Begomovirus
Tomato spotted wilt virus
double-stranded RNA
reverse transcriptase polymerase chain reaction
RNA

Cite this

Verbeek, M. ; Dullemans, A.M. ; Espino, A. ; Botella, M. ; Alfaro-Fernández, A. ; Font, M.I. / First report of southern Tomato virus in tomato in the Canary Islands, Spain. In: Journal of Plant Pathology: rivista di patologia vegetale. 2015 ; Vol. 97, No. 2. pp. 392-392.
@article{84191fed894e49aa8f001abba31f6aa5,
title = "First report of southern Tomato virus in tomato in the Canary Islands, Spain",
abstract = "In October 2006, tomato plants with torrado disease were sampled in Spain. In a sample of cv. Mariana, originating from Gran Canaria, Tomato torrado virus (ToTV, genus Torradovirus) was detected (isolate GCN06; Alfaro-Fern{\'a}ndez et al., 2010). In 2013, the sample was further analysed using next-generation sequencing (NGS). Total RNA, extracted from ca. 150 mg of leaf tissues, was used for an RNA library suitable for Illumina HiSeq paired end sequencing. A de novo assembly (using CLC Genomic Workbench v.6.5) resulted in 1380 sequence contigs. BLAST searches revealed the presence of ToTV and Tomato chlorosis virus (ToCV, genus Crinivirus). One contig (3427 nts, 8901 reads), showed 99.85{\%} identity with Southern tomato virus (STV, genus Amalgavirus), isolate Mexico-1 (GenBank accession No. EF442780), a dsRNA seed-transmitted virus (Sabanadzovic et al., 2009). Reference assembly with the Mexico-1 sequence resulted in a partial sequence of 3420 nts (GenBank KJ174690). The presence of STV in sample GCN06 was verified in RT-PCR using the specific primers STV-fw and STV-rev (Candresse et al., 2013) and sequencing of the obtained amplicon. A new sampling in May 2014 in Gran Canaria revealed the presence of STV in six out of seven tomato samples of cv Mariana. All these samples were co-infected with Pepino mosaic virus (PepMV, genus Potexvirus), Tomato spotted wilt virus (TSWV, genus Tospovirus), and Tomato yellow leaf curl virus (TYLCV, genus Begomovirus). Because STV was generally found in samples co-infected with other viruses, its symptomatology in tomato remains unclear. To our knowledge, this is the first report of STV in the Canary Islands and Spain.",
author = "M. Verbeek and A.M. Dullemans and A. Espino and M. Botella and A. Alfaro-Fern{\'a}ndez and M.I. Font",
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First report of southern Tomato virus in tomato in the Canary Islands, Spain. / Verbeek, M.; Dullemans, A.M.; Espino, A.; Botella, M.; Alfaro-Fernández, A.; Font, M.I.

In: Journal of Plant Pathology: rivista di patologia vegetale, Vol. 97, No. 2, 2015, p. 392-392.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Verbeek, M.

AU - Dullemans, A.M.

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AU - Botella, M.

AU - Alfaro-Fernández, A.

AU - Font, M.I.

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AB - In October 2006, tomato plants with torrado disease were sampled in Spain. In a sample of cv. Mariana, originating from Gran Canaria, Tomato torrado virus (ToTV, genus Torradovirus) was detected (isolate GCN06; Alfaro-Fernández et al., 2010). In 2013, the sample was further analysed using next-generation sequencing (NGS). Total RNA, extracted from ca. 150 mg of leaf tissues, was used for an RNA library suitable for Illumina HiSeq paired end sequencing. A de novo assembly (using CLC Genomic Workbench v.6.5) resulted in 1380 sequence contigs. BLAST searches revealed the presence of ToTV and Tomato chlorosis virus (ToCV, genus Crinivirus). One contig (3427 nts, 8901 reads), showed 99.85% identity with Southern tomato virus (STV, genus Amalgavirus), isolate Mexico-1 (GenBank accession No. EF442780), a dsRNA seed-transmitted virus (Sabanadzovic et al., 2009). Reference assembly with the Mexico-1 sequence resulted in a partial sequence of 3420 nts (GenBank KJ174690). The presence of STV in sample GCN06 was verified in RT-PCR using the specific primers STV-fw and STV-rev (Candresse et al., 2013) and sequencing of the obtained amplicon. A new sampling in May 2014 in Gran Canaria revealed the presence of STV in six out of seven tomato samples of cv Mariana. All these samples were co-infected with Pepino mosaic virus (PepMV, genus Potexvirus), Tomato spotted wilt virus (TSWV, genus Tospovirus), and Tomato yellow leaf curl virus (TYLCV, genus Begomovirus). Because STV was generally found in samples co-infected with other viruses, its symptomatology in tomato remains unclear. To our knowledge, this is the first report of STV in the Canary Islands and Spain.

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