First Report of Root-Knot Nematode Meloidogyne enterolobii on Tomato and Cucumber in Switzerland

S. Kiewnick, G. Karssen, J.A. Brito, M. Oggenfuss, J.E. Frey

Research output: Contribution to journalComment/Letter to the editorAcademic

19 Citations (Scopus)

Abstract

Severe stunting and extensive root galling were observed on tomato rootstock (Solanum lycopersicum L. cv. Maxifort) resistant to Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949, M. javanica (Treub, 1885), and M. arenaria (Neal, 1889) Chitwood, 1949 and cucumber (Cucumis sativus L. cv. Loustik) from two commercial greenhouses in the cantons Aargau and Lucerne in northern Switzerland. Examination of the roots of infected plants revealed the presence of root-knot nematodes in large numbers. Juveniles, males, and females were isolated, and the species was determined on the basis of morphological characteristics, including the female perineal pattern. Identification was confirmed by female esterase (Est) and malate dehydrogenase (MdH) electrophoresis (20 each for Est and MdH). All methods of identification were consistent with M. enterolobii Yang & Eisenback, 1983 (4). For further confirmation, type material of M. enterolobii (from the original host Enterolobium contortisiliquum (Vell.) Morong) from China (4) was used. Furthermore, comparison of the sequence data from 12 individuals of each of the two Swiss populations and the type material of a 310-bp fragment of cytochrome oxidase I (COI), a 723-bp fragment covering the internal transcribed spacer (ITS) region 1, 5.8s, ITS2, and part of the 26s, the mtDNA 63-bp repeat region, and a 780-bp fragment of the intergenic spacer region (1¿3) showed 100% homology and confirmed the identification as M. enterolobii. The species M. enterolobii is of great importance because it is able to reproduce on resistant tobacco, pepper, watermelon, and tomato (4). To our knowledge, this is the first report of M. enterolobii in Switzerland.
Original languageEnglish
Pages (from-to)1370-1370
JournalPlant Disease
Volume92
Issue number9
DOIs
Publication statusPublished - 2008

Fingerprint

Meloidogyne
root-knot nematodes
Switzerland
cucumbers
malate dehydrogenase
tomatoes
esterases
Enterolobium contortisiliquum
Meloidogyne arenaria
Meloidogyne javanica
Meloidogyne incognita
watermelons
Solanum lycopersicum
Cucumis sativus
intergenic DNA
pepper
cytochrome-c oxidase
growth retardation
internal transcribed spacers
electrophoresis

Cite this

Kiewnick, S. ; Karssen, G. ; Brito, J.A. ; Oggenfuss, M. ; Frey, J.E. / First Report of Root-Knot Nematode Meloidogyne enterolobii on Tomato and Cucumber in Switzerland. In: Plant Disease. 2008 ; Vol. 92, No. 9. pp. 1370-1370.
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title = "First Report of Root-Knot Nematode Meloidogyne enterolobii on Tomato and Cucumber in Switzerland",
abstract = "Severe stunting and extensive root galling were observed on tomato rootstock (Solanum lycopersicum L. cv. Maxifort) resistant to Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949, M. javanica (Treub, 1885), and M. arenaria (Neal, 1889) Chitwood, 1949 and cucumber (Cucumis sativus L. cv. Loustik) from two commercial greenhouses in the cantons Aargau and Lucerne in northern Switzerland. Examination of the roots of infected plants revealed the presence of root-knot nematodes in large numbers. Juveniles, males, and females were isolated, and the species was determined on the basis of morphological characteristics, including the female perineal pattern. Identification was confirmed by female esterase (Est) and malate dehydrogenase (MdH) electrophoresis (20 each for Est and MdH). All methods of identification were consistent with M. enterolobii Yang & Eisenback, 1983 (4). For further confirmation, type material of M. enterolobii (from the original host Enterolobium contortisiliquum (Vell.) Morong) from China (4) was used. Furthermore, comparison of the sequence data from 12 individuals of each of the two Swiss populations and the type material of a 310-bp fragment of cytochrome oxidase I (COI), a 723-bp fragment covering the internal transcribed spacer (ITS) region 1, 5.8s, ITS2, and part of the 26s, the mtDNA 63-bp repeat region, and a 780-bp fragment of the intergenic spacer region (1¿3) showed 100{\%} homology and confirmed the identification as M. enterolobii. The species M. enterolobii is of great importance because it is able to reproduce on resistant tobacco, pepper, watermelon, and tomato (4). To our knowledge, this is the first report of M. enterolobii in Switzerland.",
author = "S. Kiewnick and G. Karssen and J.A. Brito and M. Oggenfuss and J.E. Frey",
year = "2008",
doi = "10.1094/PDIS-92-9-1370A",
language = "English",
volume = "92",
pages = "1370--1370",
journal = "Plant Disease",
issn = "0191-2917",
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First Report of Root-Knot Nematode Meloidogyne enterolobii on Tomato and Cucumber in Switzerland. / Kiewnick, S.; Karssen, G.; Brito, J.A.; Oggenfuss, M.; Frey, J.E.

In: Plant Disease, Vol. 92, No. 9, 2008, p. 1370-1370.

Research output: Contribution to journalComment/Letter to the editorAcademic

TY - JOUR

T1 - First Report of Root-Knot Nematode Meloidogyne enterolobii on Tomato and Cucumber in Switzerland

AU - Kiewnick, S.

AU - Karssen, G.

AU - Brito, J.A.

AU - Oggenfuss, M.

AU - Frey, J.E.

PY - 2008

Y1 - 2008

N2 - Severe stunting and extensive root galling were observed on tomato rootstock (Solanum lycopersicum L. cv. Maxifort) resistant to Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949, M. javanica (Treub, 1885), and M. arenaria (Neal, 1889) Chitwood, 1949 and cucumber (Cucumis sativus L. cv. Loustik) from two commercial greenhouses in the cantons Aargau and Lucerne in northern Switzerland. Examination of the roots of infected plants revealed the presence of root-knot nematodes in large numbers. Juveniles, males, and females were isolated, and the species was determined on the basis of morphological characteristics, including the female perineal pattern. Identification was confirmed by female esterase (Est) and malate dehydrogenase (MdH) electrophoresis (20 each for Est and MdH). All methods of identification were consistent with M. enterolobii Yang & Eisenback, 1983 (4). For further confirmation, type material of M. enterolobii (from the original host Enterolobium contortisiliquum (Vell.) Morong) from China (4) was used. Furthermore, comparison of the sequence data from 12 individuals of each of the two Swiss populations and the type material of a 310-bp fragment of cytochrome oxidase I (COI), a 723-bp fragment covering the internal transcribed spacer (ITS) region 1, 5.8s, ITS2, and part of the 26s, the mtDNA 63-bp repeat region, and a 780-bp fragment of the intergenic spacer region (1¿3) showed 100% homology and confirmed the identification as M. enterolobii. The species M. enterolobii is of great importance because it is able to reproduce on resistant tobacco, pepper, watermelon, and tomato (4). To our knowledge, this is the first report of M. enterolobii in Switzerland.

AB - Severe stunting and extensive root galling were observed on tomato rootstock (Solanum lycopersicum L. cv. Maxifort) resistant to Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949, M. javanica (Treub, 1885), and M. arenaria (Neal, 1889) Chitwood, 1949 and cucumber (Cucumis sativus L. cv. Loustik) from two commercial greenhouses in the cantons Aargau and Lucerne in northern Switzerland. Examination of the roots of infected plants revealed the presence of root-knot nematodes in large numbers. Juveniles, males, and females were isolated, and the species was determined on the basis of morphological characteristics, including the female perineal pattern. Identification was confirmed by female esterase (Est) and malate dehydrogenase (MdH) electrophoresis (20 each for Est and MdH). All methods of identification were consistent with M. enterolobii Yang & Eisenback, 1983 (4). For further confirmation, type material of M. enterolobii (from the original host Enterolobium contortisiliquum (Vell.) Morong) from China (4) was used. Furthermore, comparison of the sequence data from 12 individuals of each of the two Swiss populations and the type material of a 310-bp fragment of cytochrome oxidase I (COI), a 723-bp fragment covering the internal transcribed spacer (ITS) region 1, 5.8s, ITS2, and part of the 26s, the mtDNA 63-bp repeat region, and a 780-bp fragment of the intergenic spacer region (1¿3) showed 100% homology and confirmed the identification as M. enterolobii. The species M. enterolobii is of great importance because it is able to reproduce on resistant tobacco, pepper, watermelon, and tomato (4). To our knowledge, this is the first report of M. enterolobii in Switzerland.

U2 - 10.1094/PDIS-92-9-1370A

DO - 10.1094/PDIS-92-9-1370A

M3 - Comment/Letter to the editor

VL - 92

SP - 1370

EP - 1370

JO - Plant Disease

JF - Plant Disease

SN - 0191-2917

IS - 9

ER -