First isolation of Nocardia crassostreae from Pacific oysters (Crassostrea gigas) in Europe

M.Y. Engelsma, I. Roozenburg, J.P. Joly

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    In summer 2006 an extensive mortality of Pacific oysters Crassostrea gigas occurred in Lake Grevelingen, the Netherlands. A sample of Pacific oysters was investigated for the presence of shellfish pathogens as potential causes of the mortality. Yellow-green lesions were observed in several oysters upon clinical inspection. Histopathology showed that 6 out of 36 oysters had a suspected bacterial infection, including 4 Nocardia-like infections. Two bacterial species, Vibrio aestuarianus and Nocardia crassostreae, were isolated from haemolymph samples and identified using PCR and sequencing of the 16S rRNA gene. This is the first isolation of N. crassostreae from shellfish in European waters. The near full-length 16S rRNA sequence of this Dutch Nocardia sp. isolate was identical to other known N. crassostreae isolates from the west coast of North America. The primary cause of oyster mortality was thought to be the physiological stress from environmental conditions, including prolonged high water temperatures and low oxygen levels. The multiple bacterial species isolated from the diseased Pacific oysters may have been a secondary cause.
    LanguageEnglish
    Pages229-234
    JournalDiseases of Aquatic Organisms
    Volume80
    Issue number3
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Crassostrea
    Nocardia
    Crassostrea gigas
    oysters
    shellfish
    mortality
    Vibrio aestuarianus
    histopathology
    ribosomal RNA
    lesion
    water temperature
    pathogen
    bacterial infections
    environmental conditions
    lesions (animal)
    hemolymph
    Netherlands
    oxygen
    gene
    coast

    Keywords

    • summer mortality
    • thunberg
    • bay

    Cite this

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    title = "First isolation of Nocardia crassostreae from Pacific oysters (Crassostrea gigas) in Europe",
    abstract = "In summer 2006 an extensive mortality of Pacific oysters Crassostrea gigas occurred in Lake Grevelingen, the Netherlands. A sample of Pacific oysters was investigated for the presence of shellfish pathogens as potential causes of the mortality. Yellow-green lesions were observed in several oysters upon clinical inspection. Histopathology showed that 6 out of 36 oysters had a suspected bacterial infection, including 4 Nocardia-like infections. Two bacterial species, Vibrio aestuarianus and Nocardia crassostreae, were isolated from haemolymph samples and identified using PCR and sequencing of the 16S rRNA gene. This is the first isolation of N. crassostreae from shellfish in European waters. The near full-length 16S rRNA sequence of this Dutch Nocardia sp. isolate was identical to other known N. crassostreae isolates from the west coast of North America. The primary cause of oyster mortality was thought to be the physiological stress from environmental conditions, including prolonged high water temperatures and low oxygen levels. The multiple bacterial species isolated from the diseased Pacific oysters may have been a secondary cause.",
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    author = "M.Y. Engelsma and I. Roozenburg and J.P. Joly",
    year = "2008",
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    language = "English",
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    First isolation of Nocardia crassostreae from Pacific oysters (Crassostrea gigas) in Europe. / Engelsma, M.Y.; Roozenburg, I.; Joly, J.P.

    In: Diseases of Aquatic Organisms, Vol. 80, No. 3, 2008, p. 229-234.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

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    AU - Engelsma, M.Y.

    AU - Roozenburg, I.

    AU - Joly, J.P.

    PY - 2008

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    AB - In summer 2006 an extensive mortality of Pacific oysters Crassostrea gigas occurred in Lake Grevelingen, the Netherlands. A sample of Pacific oysters was investigated for the presence of shellfish pathogens as potential causes of the mortality. Yellow-green lesions were observed in several oysters upon clinical inspection. Histopathology showed that 6 out of 36 oysters had a suspected bacterial infection, including 4 Nocardia-like infections. Two bacterial species, Vibrio aestuarianus and Nocardia crassostreae, were isolated from haemolymph samples and identified using PCR and sequencing of the 16S rRNA gene. This is the first isolation of N. crassostreae from shellfish in European waters. The near full-length 16S rRNA sequence of this Dutch Nocardia sp. isolate was identical to other known N. crassostreae isolates from the west coast of North America. The primary cause of oyster mortality was thought to be the physiological stress from environmental conditions, including prolonged high water temperatures and low oxygen levels. The multiple bacterial species isolated from the diseased Pacific oysters may have been a secondary cause.

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