TY - JOUR
T1 - First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
AU - Lütjohann, Dieter
AU - Björkhem, Ingemar
AU - Friedrichs, Silvia
AU - Kerksiek, Anja
AU - Lövgren-Sandblom, Anita
AU - Geilenkeuser, Wolf Jochen
AU - Ahrends, Robert
AU - Andrade, Isabel
AU - Ansorena, Diana
AU - Astiasarán, Iciar
AU - Baila-Rueda, Lucía
AU - Barriuso, Bianca
AU - Becker, Susen
AU - Bretillon, Lionel
AU - Browne, Richard W.
AU - Caccia, Claudio
AU - Ceglarek, Uta
AU - Cenarro, Ana
AU - Crick, Peter J.
AU - Fauler, Günter
AU - Garcia-Llatas, Guadalupe
AU - Gray, Robert
AU - Griffiths, William J.
AU - Gylling, Helena
AU - Harding, Scott
AU - Helmschrodt, Christin
AU - Iuliano, Luigi
AU - Janssen, Hans Gerd
AU - Jones, Peter
AU - Kaipiainen, Leena
AU - Kannenberg, Frank
AU - Lagarda, María Jesús
AU - Leoni, Valerio
AU - Lottenberg, Ana Maria
AU - MacKay, Dylan S.
AU - Matysik, Silke
AU - McDonald, Jeff
AU - Menendez-Carreño, Maria
AU - Myrie, Semone B.
AU - Sutti Nunes, Valéria
AU - Ostlund, Richard E.
AU - Polisecki, Eliana
AU - Ramos, Fernando
AU - Rideout, Todd C.
AU - Schaefer, Ernst J.
AU - Schmitz, Gerd
AU - Wang, Yuqin
AU - Zerbinati, Chiara
AU - Diczfalusy, Ulf
AU - Schött, Hans Frieder
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.
AB - Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.
KW - Atherosclerosis
KW - Cholesterol absorption
KW - Cholesterol balance
KW - Cholesterol synthesis
KW - Phytosterols
KW - Surrogate marker
U2 - 10.1016/j.jsbmb.2019.03.025
DO - 10.1016/j.jsbmb.2019.03.025
M3 - Article
C2 - 30940596
AN - SCOPUS:85063754878
SN - 0960-0760
VL - 190
SP - 115
EP - 125
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
ER -