First evidence of crayfish plaque agent in populations of the marbled caryfish (Procambarus fallux forma virginalis)

N.S. Keller, M. Pfeiffer, I. Roessink, R. Schulz, A. Schrimpf

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

The introduction of non-indigenous species and associated diseases can cause declines in indigenous flora and fauna and threaten local biodiversity. The crayfish plague pathogen (Aphanomyces astaci), carried and transmitted by latent infected North American crayfish, can lead to high mortalities in indigenous European crayfish populations. Although the parthenogenetic marbled crayfish (Procambarus fallax (Hagen, 1870) forma virginalis) is common in the aquarium trade and has established wild populations in Europe, its carrier status is still unknown. This study investigated one captive and three established wild-living marbled crayfish populations for an infection with the crayfish plague pathogen applying real-time PCR. We demonstrate that captive, as well as two wild marbled crayfish populations were infected by A. astaci. Although infection status in laboratory kept specimens reached high levels, marbled crayfish showed no obviously plague-related mortality. Furthermore, sequence analysis revealed that captive crayfish carried the A. astaci genotype Pc, which has earlier been isolated from the North American red swamp crayfish (Procambarus clarkii). The results indicate that due to its positive carrier status marbled crayfish poses a greater threat to local biodiversity in Europe than considered until now.
Original languageEnglish
Article number15
Number of pages8
JournalKnowledge and Management of Aquatic Ecosystems
Issue number414
DOIs
Publication statusPublished - 2014

Fingerprint

Procambarus
crayfish
Aphanomyces astaci
plague
carrier state
Procambarus clarkii
pathogen
biodiversity
mortality
pathogens
aquariums
infection
aquarium
wild population
swamp
quantitative polymerase chain reaction
sequence analysis
flora
genotype
fauna

Keywords

  • pathogen aphanomyces-astaci
  • polymerase-chain-reaction
  • north-american crayfish
  • fresh-water crayfish
  • noble crayfish
  • marmorkrebs decapoda
  • adaptation
  • diversity
  • hagen
  • cambaridae

Cite this

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title = "First evidence of crayfish plaque agent in populations of the marbled caryfish (Procambarus fallux forma virginalis)",
abstract = "The introduction of non-indigenous species and associated diseases can cause declines in indigenous flora and fauna and threaten local biodiversity. The crayfish plague pathogen (Aphanomyces astaci), carried and transmitted by latent infected North American crayfish, can lead to high mortalities in indigenous European crayfish populations. Although the parthenogenetic marbled crayfish (Procambarus fallax (Hagen, 1870) forma virginalis) is common in the aquarium trade and has established wild populations in Europe, its carrier status is still unknown. This study investigated one captive and three established wild-living marbled crayfish populations for an infection with the crayfish plague pathogen applying real-time PCR. We demonstrate that captive, as well as two wild marbled crayfish populations were infected by A. astaci. Although infection status in laboratory kept specimens reached high levels, marbled crayfish showed no obviously plague-related mortality. Furthermore, sequence analysis revealed that captive crayfish carried the A. astaci genotype Pc, which has earlier been isolated from the North American red swamp crayfish (Procambarus clarkii). The results indicate that due to its positive carrier status marbled crayfish poses a greater threat to local biodiversity in Europe than considered until now.",
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author = "N.S. Keller and M. Pfeiffer and I. Roessink and R. Schulz and A. Schrimpf",
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First evidence of crayfish plaque agent in populations of the marbled caryfish (Procambarus fallux forma virginalis). / Keller, N.S.; Pfeiffer, M.; Roessink, I.; Schulz, R.; Schrimpf, A.

In: Knowledge and Management of Aquatic Ecosystems, No. 414, 15, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - First evidence of crayfish plaque agent in populations of the marbled caryfish (Procambarus fallux forma virginalis)

AU - Keller, N.S.

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AU - Schulz, R.

AU - Schrimpf, A.

PY - 2014

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N2 - The introduction of non-indigenous species and associated diseases can cause declines in indigenous flora and fauna and threaten local biodiversity. The crayfish plague pathogen (Aphanomyces astaci), carried and transmitted by latent infected North American crayfish, can lead to high mortalities in indigenous European crayfish populations. Although the parthenogenetic marbled crayfish (Procambarus fallax (Hagen, 1870) forma virginalis) is common in the aquarium trade and has established wild populations in Europe, its carrier status is still unknown. This study investigated one captive and three established wild-living marbled crayfish populations for an infection with the crayfish plague pathogen applying real-time PCR. We demonstrate that captive, as well as two wild marbled crayfish populations were infected by A. astaci. Although infection status in laboratory kept specimens reached high levels, marbled crayfish showed no obviously plague-related mortality. Furthermore, sequence analysis revealed that captive crayfish carried the A. astaci genotype Pc, which has earlier been isolated from the North American red swamp crayfish (Procambarus clarkii). The results indicate that due to its positive carrier status marbled crayfish poses a greater threat to local biodiversity in Europe than considered until now.

AB - The introduction of non-indigenous species and associated diseases can cause declines in indigenous flora and fauna and threaten local biodiversity. The crayfish plague pathogen (Aphanomyces astaci), carried and transmitted by latent infected North American crayfish, can lead to high mortalities in indigenous European crayfish populations. Although the parthenogenetic marbled crayfish (Procambarus fallax (Hagen, 1870) forma virginalis) is common in the aquarium trade and has established wild populations in Europe, its carrier status is still unknown. This study investigated one captive and three established wild-living marbled crayfish populations for an infection with the crayfish plague pathogen applying real-time PCR. We demonstrate that captive, as well as two wild marbled crayfish populations were infected by A. astaci. Although infection status in laboratory kept specimens reached high levels, marbled crayfish showed no obviously plague-related mortality. Furthermore, sequence analysis revealed that captive crayfish carried the A. astaci genotype Pc, which has earlier been isolated from the North American red swamp crayfish (Procambarus clarkii). The results indicate that due to its positive carrier status marbled crayfish poses a greater threat to local biodiversity in Europe than considered until now.

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KW - adaptation

KW - diversity

KW - hagen

KW - cambaridae

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