First characterization of bioactive components in soybean tempe that protect human and animal intestinal cells against enterotoxigenic Escherichia coli (ETEC) infection

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Tempe extracts can inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to intestinal cells and thereby can play a role in controlling ETEC-induced diarrhea. The component responsible for this adhesion inhibition activity is still unknown. This research describes the purification and partial characterization of this bioactive component of tempe. After heating, defatting, and protease treatment, the extracts were found to remain active. However, after treatment with polysaccharide-degrading enzyme mixtures the bioactivity was lost. Ultrafiltration revealed the active component to be >30 kDa. Further purification of the bioactive tempe extracts yielded an active fraction with an increased carbohydrate content of higher arabinose content than the nonactive fractions. In conclusion, the bioactive component contains arabinose and originates from the arabinan or arabinogalactan side chain of the pectic cell wall polysaccharides of the soybeans, which is probably released or formed during fermentation by enzymatic modifications
Original languageEnglish
Pages (from-to)7649-7656
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number13
DOIs
Publication statusPublished - 2010

Keywords

  • soya bean tempe
  • aspergillus-niger
  • adhesion
  • fermentation
  • inhibition
  • diarrhea
  • piglets
  • k88
  • polysaccharides
  • arabinanases

Fingerprint Dive into the research topics of 'First characterization of bioactive components in soybean tempe that protect human and animal intestinal cells against enterotoxigenic Escherichia coli (ETEC) infection'. Together they form a unique fingerprint.

Cite this