Fine mapping of two major QTLs conferring resistance to powdery mildew in tomato

L. Faino, S. Azizinia, B. Houshyani Hassanzadeh, E.C. Verzaux, M.R. Ercolano, R.G.F. Visser, Y. Bai

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9 Citations (Scopus)

Abstract

Tomato (Solanum lycopersicum) is the most cultivated crop in the Solanaceae family and is a host for Oidium neolycopersici, the cause agent of powdery mildew disease. In wild species of tomato, genes (Ol-1–Ol-6) for monogenic resistance have been identified. Moreover, three quantitative resistance loci (QRLs), namely Ol-qtl1, Ol-qtl2 and Ol-qtl3, have been mapped in Solanum neorickii G1.1601. In this work, we developed several advanced backcross populations in order to fine-map these Ol-qtls. Resistant lines harboring individual Ol-qtl were produced and used in recombinant screening. Ten recombinants were identified in chromosomal regions carrying Ol-qtl1s. The recombinant individuals were used to produce recombinant families (RFs). By screening these RFs with molecular markers and testing them with O. neolycopersici, we could localize Ol-qtl1 in a region of about 2.3 Mbp on the long arm of chromosome 6 and Ol-qtl2 in a region of 2.5 Mbp on the short arm of chromosome 12. On the other hand, the presence of Ol-qtl3 locus was not confirmed in this study. The fine-mapping results further demonstrated the co-localization between Ol-qtls and genes for monogenic resistance; the Ol-qtl1 interval contains the Ol-1 gene and the Ol-qtl2 interval harbors the Lv gene that confers monogenic resistance to Leveillula taurica, another species of tomato powdery mildew.
Original languageEnglish
Pages (from-to)223-234
JournalEuphytica
Volume184
Issue number2
DOIs
Publication statusPublished - 2012

Keywords

  • quantitative trait loci
  • disease resistance
  • oidium-neolycopersici
  • phytophthora-infestans
  • dependent resistance
  • plant defense
  • genetic-map
  • late blight
  • linkage
  • potato

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