Fine mapping of a major QTL for awn length in barley using a multiparent mapping population

Corinna B. Liller, Agatha Walla, Martin P. Boer, Pete Hedley, Malcolm Macaulay, Sieglinde Effgen, Maria von Korff, G.W. van Esse, Maarten Koornneef

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Key message: Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq.Abstract: Barley awns are photosynthetically active and contribute to grain yield. Awn length is variable among both wild and cultivated barley genotypes and many mutants with alterations in awn length have been identified. Here, we used a multiparent mapping population derived from cv. Morex and four genetically diverse wild barley lines to detect quantitative trait loci (QTLs) for awn length. Twelve QTLs, distributed over the barley genome, were identified with the most significant one located on chromosome arm 7HL (QTL AL7.1). The effect of AL7.1 was confirmed using near isogenic lines (NILs) and fine-mapped in two independent heterogeneous inbred families to a <0.9 cM interval. With exception of a small effect on grain width, no other traits such as plant height or flowering time were affected by AL7.1. Variant calling on transcripts obtained from RNA sequencing reads in NILs was used to narrow down the list of candidate genes located in the interval. This data may be used for further characterization and unravelling of the mechanisms underlying natural variation in awn length.

Original languageEnglish
Pages (from-to)269-281
JournalTheoretical and Applied Genetics
Volume130
Issue number2
DOIs
Publication statusPublished - 2017

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Quantitative Trait Loci
Hordeum
quantitative trait loci
barley
isogenic lines
Population
RNA Sequence Analysis
Genes
Chromosomes
Genotype
Genome
RNA
genes
sequence analysis
grain yield
flowering
chromosomes
mutants
genome
genotype

Cite this

Liller, Corinna B. ; Walla, Agatha ; Boer, Martin P. ; Hedley, Pete ; Macaulay, Malcolm ; Effgen, Sieglinde ; von Korff, Maria ; van Esse, G.W. ; Koornneef, Maarten. / Fine mapping of a major QTL for awn length in barley using a multiparent mapping population. In: Theoretical and Applied Genetics. 2017 ; Vol. 130, No. 2. pp. 269-281.
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abstract = "Key message: Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq.Abstract: Barley awns are photosynthetically active and contribute to grain yield. Awn length is variable among both wild and cultivated barley genotypes and many mutants with alterations in awn length have been identified. Here, we used a multiparent mapping population derived from cv. Morex and four genetically diverse wild barley lines to detect quantitative trait loci (QTLs) for awn length. Twelve QTLs, distributed over the barley genome, were identified with the most significant one located on chromosome arm 7HL (QTL AL7.1). The effect of AL7.1 was confirmed using near isogenic lines (NILs) and fine-mapped in two independent heterogeneous inbred families to a <0.9 cM interval. With exception of a small effect on grain width, no other traits such as plant height or flowering time were affected by AL7.1. Variant calling on transcripts obtained from RNA sequencing reads in NILs was used to narrow down the list of candidate genes located in the interval. This data may be used for further characterization and unravelling of the mechanisms underlying natural variation in awn length.",
author = "Liller, {Corinna B.} and Agatha Walla and Boer, {Martin P.} and Pete Hedley and Malcolm Macaulay and Sieglinde Effgen and {von Korff}, Maria and {van Esse}, G.W. and Maarten Koornneef",
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Liller, CB, Walla, A, Boer, MP, Hedley, P, Macaulay, M, Effgen, S, von Korff, M, van Esse, GW & Koornneef, M 2017, 'Fine mapping of a major QTL for awn length in barley using a multiparent mapping population', Theoretical and Applied Genetics, vol. 130, no. 2, pp. 269-281. https://doi.org/10.1007/s00122-016-2807-y

Fine mapping of a major QTL for awn length in barley using a multiparent mapping population. / Liller, Corinna B.; Walla, Agatha; Boer, Martin P.; Hedley, Pete; Macaulay, Malcolm; Effgen, Sieglinde; von Korff, Maria; van Esse, G.W.; Koornneef, Maarten.

In: Theoretical and Applied Genetics, Vol. 130, No. 2, 2017, p. 269-281.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Liller, Corinna B.

AU - Walla, Agatha

AU - Boer, Martin P.

AU - Hedley, Pete

AU - Macaulay, Malcolm

AU - Effgen, Sieglinde

AU - von Korff, Maria

AU - van Esse, G.W.

AU - Koornneef, Maarten

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N2 - Key message: Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq.Abstract: Barley awns are photosynthetically active and contribute to grain yield. Awn length is variable among both wild and cultivated barley genotypes and many mutants with alterations in awn length have been identified. Here, we used a multiparent mapping population derived from cv. Morex and four genetically diverse wild barley lines to detect quantitative trait loci (QTLs) for awn length. Twelve QTLs, distributed over the barley genome, were identified with the most significant one located on chromosome arm 7HL (QTL AL7.1). The effect of AL7.1 was confirmed using near isogenic lines (NILs) and fine-mapped in two independent heterogeneous inbred families to a <0.9 cM interval. With exception of a small effect on grain width, no other traits such as plant height or flowering time were affected by AL7.1. Variant calling on transcripts obtained from RNA sequencing reads in NILs was used to narrow down the list of candidate genes located in the interval. This data may be used for further characterization and unravelling of the mechanisms underlying natural variation in awn length.

AB - Key message: Awn length was mapped using a multiparent population derived from cv. Morex and four wild accessions. One QTL was fine mapped and candidate genes were identified in NILs by RNA-seq.Abstract: Barley awns are photosynthetically active and contribute to grain yield. Awn length is variable among both wild and cultivated barley genotypes and many mutants with alterations in awn length have been identified. Here, we used a multiparent mapping population derived from cv. Morex and four genetically diverse wild barley lines to detect quantitative trait loci (QTLs) for awn length. Twelve QTLs, distributed over the barley genome, were identified with the most significant one located on chromosome arm 7HL (QTL AL7.1). The effect of AL7.1 was confirmed using near isogenic lines (NILs) and fine-mapped in two independent heterogeneous inbred families to a <0.9 cM interval. With exception of a small effect on grain width, no other traits such as plant height or flowering time were affected by AL7.1. Variant calling on transcripts obtained from RNA sequencing reads in NILs was used to narrow down the list of candidate genes located in the interval. This data may be used for further characterization and unravelling of the mechanisms underlying natural variation in awn length.

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DO - 10.1007/s00122-016-2807-y

M3 - Article

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SP - 269

EP - 281

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

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ER -