Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia

H.J.J. Koehorst-van Putten, E. Sudarmonowati, M. Herman, I.J. Pereira-Bertram, A.M.A. Wolters, H. Meima, N. de Vetten, C.J.J.M. Raemakers, R.G.F. Visser

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

The development and testing in the field of genetically modified -so called- orphan crops like cassava in tropical countries is still in its infancy, despite the fact that cassava is not only used for food and feed but is also an important industrial crop. As traditional breeding of cassava is difficult (allodiploid, vegetatively propagated, outbreeding species) it is an ideal crop for improvement through genetic modification. We here report on the results of production and field testing of genetically modified low-amylose transformants of commercial cassava variety Adira4 in Indonesia. Twenty four transformants were produced and selected in the Netherlands based on phenotypic and molecular analyses. Nodal cuttings of these plants were sent to Indonesia where they were grown under biosafety conditions. After two screenhouse tests 15 transformants remained for a field trial. The tuberous root yield of 10 transformants was not significantly different from the control. Starch from transformants in which amylose was very low or absent showed all physical and rheological properties as expected from amylose-free cassava starch. The improved functionality of the starch was shown for an adipate acetate starch which was made into a tomato sauce. This is the first account of a field trial with transgenic cassava which shows that by using genetic modification it is possible to obtain low-amylose cassava plants with commercial potential with good root yield and starch quality.
Original languageEnglish
Pages (from-to)39-50
JournalTransgenic Research
Volume21
Issue number1
DOIs
Publication statusPublished - 2012

Fingerprint

Manihot
Amylose
Indonesia
amylose
cassava
Starch
starch
testing
genetic engineering
field experimentation
tomato sauce
plant cuttings
industrial crops
cassava starch
outbreeding
biosafety
infancy
crops
rheological properties
Lycopersicon esculentum

Keywords

  • manihot-esculenta crantz
  • cyclic somatic embryogenesis
  • t-dna
  • potato
  • transformation
  • plants
  • regeneration
  • expression
  • synthase
  • cultures

Cite this

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title = "Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia",
abstract = "The development and testing in the field of genetically modified -so called- orphan crops like cassava in tropical countries is still in its infancy, despite the fact that cassava is not only used for food and feed but is also an important industrial crop. As traditional breeding of cassava is difficult (allodiploid, vegetatively propagated, outbreeding species) it is an ideal crop for improvement through genetic modification. We here report on the results of production and field testing of genetically modified low-amylose transformants of commercial cassava variety Adira4 in Indonesia. Twenty four transformants were produced and selected in the Netherlands based on phenotypic and molecular analyses. Nodal cuttings of these plants were sent to Indonesia where they were grown under biosafety conditions. After two screenhouse tests 15 transformants remained for a field trial. The tuberous root yield of 10 transformants was not significantly different from the control. Starch from transformants in which amylose was very low or absent showed all physical and rheological properties as expected from amylose-free cassava starch. The improved functionality of the starch was shown for an adipate acetate starch which was made into a tomato sauce. This is the first account of a field trial with transgenic cassava which shows that by using genetic modification it is possible to obtain low-amylose cassava plants with commercial potential with good root yield and starch quality.",
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author = "{Koehorst-van Putten}, H.J.J. and E. Sudarmonowati and M. Herman and I.J. Pereira-Bertram and A.M.A. Wolters and H. Meima and {de Vetten}, N. and C.J.J.M. Raemakers and R.G.F. Visser",
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Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia. / Koehorst-van Putten, H.J.J.; Sudarmonowati, E.; Herman, M.; Pereira-Bertram, I.J.; Wolters, A.M.A.; Meima, H.; de Vetten, N.; Raemakers, C.J.J.M.; Visser, R.G.F.

In: Transgenic Research, Vol. 21, No. 1, 2012, p. 39-50.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia

AU - Koehorst-van Putten, H.J.J.

AU - Sudarmonowati, E.

AU - Herman, M.

AU - Pereira-Bertram, I.J.

AU - Wolters, A.M.A.

AU - Meima, H.

AU - de Vetten, N.

AU - Raemakers, C.J.J.M.

AU - Visser, R.G.F.

PY - 2012

Y1 - 2012

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AB - The development and testing in the field of genetically modified -so called- orphan crops like cassava in tropical countries is still in its infancy, despite the fact that cassava is not only used for food and feed but is also an important industrial crop. As traditional breeding of cassava is difficult (allodiploid, vegetatively propagated, outbreeding species) it is an ideal crop for improvement through genetic modification. We here report on the results of production and field testing of genetically modified low-amylose transformants of commercial cassava variety Adira4 in Indonesia. Twenty four transformants were produced and selected in the Netherlands based on phenotypic and molecular analyses. Nodal cuttings of these plants were sent to Indonesia where they were grown under biosafety conditions. After two screenhouse tests 15 transformants remained for a field trial. The tuberous root yield of 10 transformants was not significantly different from the control. Starch from transformants in which amylose was very low or absent showed all physical and rheological properties as expected from amylose-free cassava starch. The improved functionality of the starch was shown for an adipate acetate starch which was made into a tomato sauce. This is the first account of a field trial with transgenic cassava which shows that by using genetic modification it is possible to obtain low-amylose cassava plants with commercial potential with good root yield and starch quality.

KW - manihot-esculenta crantz

KW - cyclic somatic embryogenesis

KW - t-dna

KW - potato

KW - transformation

KW - plants

KW - regeneration

KW - expression

KW - synthase

KW - cultures

U2 - 10.1007/s11248-011-9507-9

DO - 10.1007/s11248-011-9507-9

M3 - Article

VL - 21

SP - 39

EP - 50

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 1

ER -