Fibulorhizoctonia psychrophila is the causal agent of lenticel spot on apple and pear fruit in the Netherlands

M. Wenneker*, K.T.K. Pham, M.E.C. Lemmers, F.A. de Boer, P.J. van Leeuwen, T.C. Hollinger, F.G. van de Geijn, B.P.H.J. Thomma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

In a survey for postharvest diseases of apples and pears in the Netherlands, an unknown postharvest fruit rot was observed. The disease appeared to originate from infected lenticels. A fungus was consistently isolated from the decayed fruits. The fungal pathogen was isolated on potato dextrose agar, and at low temperatures development of a fast-growing whitish mycelium was observed. Growth of this fungus was observed between 1 and 20 °C with an optimum at 15 °C, while incubation of mycelium at 25 °C resulted in no growth. The isolates did not produce asexual or sexual spores. The isolates were characterized and identified by morphology and molecular phylogenetic analysis. Genomic DNA was isolated and amplified using ITS1-ITS4, EF1 and RPB2 primers, and BLAST searches in GenBank placed the fungus taxonomically in the genus Fibulorhizoctonia, with the highest matches to F. psychrophila. Pathogenicity of representative isolates from apple and pear fruit was confirmed under laboratory conditions. To the best of our knowledge this is the first report of F. psychrophila causing lenticel spot on apple and pear, and also the cause of a whitish mould on storage bins.

Original languageEnglish
Number of pages5
JournalEuropean Journal of Plant Pathology
DOIs
Publication statusPublished - 7 Oct 2016

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pears
Netherlands
apples
mycelium
fungi
fruits
bins (containers)
postharvest diseases
molds (fungi)
pathogenicity
spores
agar
potatoes
genomics
glucose
pathogens
phylogeny
DNA
temperature

Keywords

  • Athelia
  • Postharvest

Cite this

@article{2ff43b726b9a4d4ea93e0f8e02b5f609,
title = "Fibulorhizoctonia psychrophila is the causal agent of lenticel spot on apple and pear fruit in the Netherlands",
abstract = "In a survey for postharvest diseases of apples and pears in the Netherlands, an unknown postharvest fruit rot was observed. The disease appeared to originate from infected lenticels. A fungus was consistently isolated from the decayed fruits. The fungal pathogen was isolated on potato dextrose agar, and at low temperatures development of a fast-growing whitish mycelium was observed. Growth of this fungus was observed between 1 and 20 °C with an optimum at 15 °C, while incubation of mycelium at 25 °C resulted in no growth. The isolates did not produce asexual or sexual spores. The isolates were characterized and identified by morphology and molecular phylogenetic analysis. Genomic DNA was isolated and amplified using ITS1-ITS4, EF1 and RPB2 primers, and BLAST searches in GenBank placed the fungus taxonomically in the genus Fibulorhizoctonia, with the highest matches to F. psychrophila. Pathogenicity of representative isolates from apple and pear fruit was confirmed under laboratory conditions. To the best of our knowledge this is the first report of F. psychrophila causing lenticel spot on apple and pear, and also the cause of a whitish mould on storage bins.",
keywords = "Athelia, Postharvest",
author = "M. Wenneker and K.T.K. Pham and M.E.C. Lemmers and {de Boer}, F.A. and {van Leeuwen}, P.J. and T.C. Hollinger and {van de Geijn}, F.G. and B.P.H.J. Thomma",
year = "2016",
month = "10",
day = "7",
doi = "10.1007/s10658-016-1061-0",
language = "English",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
publisher = "Springer Verlag",

}

Fibulorhizoctonia psychrophila is the causal agent of lenticel spot on apple and pear fruit in the Netherlands. / Wenneker, M.; Pham, K.T.K.; Lemmers, M.E.C.; de Boer, F.A.; van Leeuwen, P.J.; Hollinger, T.C.; van de Geijn, F.G.; Thomma, B.P.H.J.

In: European Journal of Plant Pathology, 07.10.2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Fibulorhizoctonia psychrophila is the causal agent of lenticel spot on apple and pear fruit in the Netherlands

AU - Wenneker, M.

AU - Pham, K.T.K.

AU - Lemmers, M.E.C.

AU - de Boer, F.A.

AU - van Leeuwen, P.J.

AU - Hollinger, T.C.

AU - van de Geijn, F.G.

AU - Thomma, B.P.H.J.

PY - 2016/10/7

Y1 - 2016/10/7

N2 - In a survey for postharvest diseases of apples and pears in the Netherlands, an unknown postharvest fruit rot was observed. The disease appeared to originate from infected lenticels. A fungus was consistently isolated from the decayed fruits. The fungal pathogen was isolated on potato dextrose agar, and at low temperatures development of a fast-growing whitish mycelium was observed. Growth of this fungus was observed between 1 and 20 °C with an optimum at 15 °C, while incubation of mycelium at 25 °C resulted in no growth. The isolates did not produce asexual or sexual spores. The isolates were characterized and identified by morphology and molecular phylogenetic analysis. Genomic DNA was isolated and amplified using ITS1-ITS4, EF1 and RPB2 primers, and BLAST searches in GenBank placed the fungus taxonomically in the genus Fibulorhizoctonia, with the highest matches to F. psychrophila. Pathogenicity of representative isolates from apple and pear fruit was confirmed under laboratory conditions. To the best of our knowledge this is the first report of F. psychrophila causing lenticel spot on apple and pear, and also the cause of a whitish mould on storage bins.

AB - In a survey for postharvest diseases of apples and pears in the Netherlands, an unknown postharvest fruit rot was observed. The disease appeared to originate from infected lenticels. A fungus was consistently isolated from the decayed fruits. The fungal pathogen was isolated on potato dextrose agar, and at low temperatures development of a fast-growing whitish mycelium was observed. Growth of this fungus was observed between 1 and 20 °C with an optimum at 15 °C, while incubation of mycelium at 25 °C resulted in no growth. The isolates did not produce asexual or sexual spores. The isolates were characterized and identified by morphology and molecular phylogenetic analysis. Genomic DNA was isolated and amplified using ITS1-ITS4, EF1 and RPB2 primers, and BLAST searches in GenBank placed the fungus taxonomically in the genus Fibulorhizoctonia, with the highest matches to F. psychrophila. Pathogenicity of representative isolates from apple and pear fruit was confirmed under laboratory conditions. To the best of our knowledge this is the first report of F. psychrophila causing lenticel spot on apple and pear, and also the cause of a whitish mould on storage bins.

KW - Athelia

KW - Postharvest

U2 - 10.1007/s10658-016-1061-0

DO - 10.1007/s10658-016-1061-0

M3 - Article

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

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