Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine

Consolacion Garcia Contreras, M. Vazquez-Gomez, O. Madsen, M. Groenen, S. Astiz, Y. Nunez, R. Benitez, A. Heras-Molina, A. Fernandez, B. Isabel, A.I. Rey, A. Gonzalez-Bulnes, C. Ovilo

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Iberian pigs are more prone to obesity and metabolic alterations than other lean swine breeds, due to leptin resistance and thrifty genotype, which makes the breed susceptible to changes in amount and composition of diet. This is especially manifested during prenatal development, where nutritional deficiencies in the diet of the mother may modify offspring’s gene expression to cope with postnatal challenging conditions. This work aimed to assess the role of the genotype in the metabolic and transcriptomic changes of pig fetuses derived from undernourished pregnant sows. Pure Iberian sows were inseminated with heterospermic semen from Iberian and Large White boars to obtain IBxIB and IBxLW genotypes. The pregnancy was challenged by diminishingmaternal intake to 50% of requirements during the last 2 thirds of pregnancy. Fifty-one fetuses were obtained at Day 77 of pregnancy and 32 of them (16 from each genotype) were selected at random. Assessment of body traits showed that IBxLW fetuses were heavier and more corpulent than their IBxIB littermates (P < 0.05). Analysis of liver fatty acids (FA) showed that IBxIB had higher level of saturated FA and monounsaturated FA and lower levels of polyunsaturated FA than IBxLW fetuses (P < 0.0001). Liver transcriptome analysis resulted in 249 genes differentially expressed (DE) between the 2 genotypes (q < 0.05 and FC >1.3). Functional annotation of the DE genes revealed a downregulation of biological functions related to growth and development (e.g.: Cell survival, Cell movement, Quantity of connective tissue or Survival of organism) in IBxIB fetuses. Potential transcription factors were assessed with IPA software, which allow the prediction of upstream regulators that may explain the molecular mechanisms responsible of transcriptome differences between genetic types. We highlighted 955 potential upstream regulators, some of them being involvedin growth and development (e.g.: IGF1) and downregulated in IBxIB fetuses. Hence, our results indicate differential regulation of relevant functions and pathways involved in growth and development, which may explain the phenotypic differences among the fatty and lean swine genotypes.
Original languageEnglish
Title of host publicationISAG 2019 Abstract Book
Subtitle of host publication37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain
Pages148-149
Publication statusPublished - 7 Jul 2019
Event37th International Society for Animal Genetics Conference (ISAG) - Lleida, Spain
Duration: 7 Jul 201912 Jul 2019

Conference

Conference37th International Society for Animal Genetics Conference (ISAG)
CountrySpain
CityLleida
Period7/07/1912/07/19

Fingerprint

transcriptomics
fetus
fatty acid composition
swine
genotype
growth and development
pregnancy
sows
prenatal development
swine breeds
Large White
nutrient deficiencies
leptin
boars
cell movement
transcriptome
diet
connective tissues
cell viability
semen

Cite this

Garcia Contreras, C., Vazquez-Gomez, M., Madsen, O., Groenen, M., Astiz, S., Nunez, Y., ... Ovilo, C. (2019). Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine. In ISAG 2019 Abstract Book: 37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain (pp. 148-149)
Garcia Contreras, Consolacion ; Vazquez-Gomez, M. ; Madsen, O. ; Groenen, M. ; Astiz, S. ; Nunez, Y. ; Benitez, R. ; Heras-Molina, A. ; Fernandez, A. ; Isabel, B. ; Rey, A.I. ; Gonzalez-Bulnes, A. ; Ovilo, C. / Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine. ISAG 2019 Abstract Book: 37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain. 2019. pp. 148-149
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abstract = "Iberian pigs are more prone to obesity and metabolic alterations than other lean swine breeds, due to leptin resistance and thrifty genotype, which makes the breed susceptible to changes in amount and composition of diet. This is especially manifested during prenatal development, where nutritional deficiencies in the diet of the mother may modify offspring’s gene expression to cope with postnatal challenging conditions. This work aimed to assess the role of the genotype in the metabolic and transcriptomic changes of pig fetuses derived from undernourished pregnant sows. Pure Iberian sows were inseminated with heterospermic semen from Iberian and Large White boars to obtain IBxIB and IBxLW genotypes. The pregnancy was challenged by diminishingmaternal intake to 50{\%} of requirements during the last 2 thirds of pregnancy. Fifty-one fetuses were obtained at Day 77 of pregnancy and 32 of them (16 from each genotype) were selected at random. Assessment of body traits showed that IBxLW fetuses were heavier and more corpulent than their IBxIB littermates (P < 0.05). Analysis of liver fatty acids (FA) showed that IBxIB had higher level of saturated FA and monounsaturated FA and lower levels of polyunsaturated FA than IBxLW fetuses (P < 0.0001). Liver transcriptome analysis resulted in 249 genes differentially expressed (DE) between the 2 genotypes (q < 0.05 and FC >1.3). Functional annotation of the DE genes revealed a downregulation of biological functions related to growth and development (e.g.: Cell survival, Cell movement, Quantity of connective tissue or Survival of organism) in IBxIB fetuses. Potential transcription factors were assessed with IPA software, which allow the prediction of upstream regulators that may explain the molecular mechanisms responsible of transcriptome differences between genetic types. We highlighted 955 potential upstream regulators, some of them being involvedin growth and development (e.g.: IGF1) and downregulated in IBxIB fetuses. Hence, our results indicate differential regulation of relevant functions and pathways involved in growth and development, which may explain the phenotypic differences among the fatty and lean swine genotypes.",
author = "{Garcia Contreras}, Consolacion and M. Vazquez-Gomez and O. Madsen and M. Groenen and S. Astiz and Y. Nunez and R. Benitez and A. Heras-Molina and A. Fernandez and B. Isabel and A.I. Rey and A. Gonzalez-Bulnes and C. Ovilo",
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Garcia Contreras, C, Vazquez-Gomez, M, Madsen, O, Groenen, M, Astiz, S, Nunez, Y, Benitez, R, Heras-Molina, A, Fernandez, A, Isabel, B, Rey, AI, Gonzalez-Bulnes, A & Ovilo, C 2019, Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine. in ISAG 2019 Abstract Book: 37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain. pp. 148-149, Lleida, Spain, 7/07/19.

Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine. / Garcia Contreras, Consolacion; Vazquez-Gomez, M.; Madsen, O.; Groenen, M.; Astiz, S.; Nunez, Y.; Benitez, R.; Heras-Molina, A.; Fernandez, A.; Isabel, B.; Rey, A.I.; Gonzalez-Bulnes, A.; Ovilo, C.

ISAG 2019 Abstract Book: 37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain. 2019. p. 148-149.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine

AU - Garcia Contreras, Consolacion

AU - Vazquez-Gomez, M.

AU - Madsen, O.

AU - Groenen, M.

AU - Astiz, S.

AU - Nunez, Y.

AU - Benitez, R.

AU - Heras-Molina, A.

AU - Fernandez, A.

AU - Isabel, B.

AU - Rey, A.I.

AU - Gonzalez-Bulnes, A.

AU - Ovilo, C.

PY - 2019/7/7

Y1 - 2019/7/7

N2 - Iberian pigs are more prone to obesity and metabolic alterations than other lean swine breeds, due to leptin resistance and thrifty genotype, which makes the breed susceptible to changes in amount and composition of diet. This is especially manifested during prenatal development, where nutritional deficiencies in the diet of the mother may modify offspring’s gene expression to cope with postnatal challenging conditions. This work aimed to assess the role of the genotype in the metabolic and transcriptomic changes of pig fetuses derived from undernourished pregnant sows. Pure Iberian sows were inseminated with heterospermic semen from Iberian and Large White boars to obtain IBxIB and IBxLW genotypes. The pregnancy was challenged by diminishingmaternal intake to 50% of requirements during the last 2 thirds of pregnancy. Fifty-one fetuses were obtained at Day 77 of pregnancy and 32 of them (16 from each genotype) were selected at random. Assessment of body traits showed that IBxLW fetuses were heavier and more corpulent than their IBxIB littermates (P < 0.05). Analysis of liver fatty acids (FA) showed that IBxIB had higher level of saturated FA and monounsaturated FA and lower levels of polyunsaturated FA than IBxLW fetuses (P < 0.0001). Liver transcriptome analysis resulted in 249 genes differentially expressed (DE) between the 2 genotypes (q < 0.05 and FC >1.3). Functional annotation of the DE genes revealed a downregulation of biological functions related to growth and development (e.g.: Cell survival, Cell movement, Quantity of connective tissue or Survival of organism) in IBxIB fetuses. Potential transcription factors were assessed with IPA software, which allow the prediction of upstream regulators that may explain the molecular mechanisms responsible of transcriptome differences between genetic types. We highlighted 955 potential upstream regulators, some of them being involvedin growth and development (e.g.: IGF1) and downregulated in IBxIB fetuses. Hence, our results indicate differential regulation of relevant functions and pathways involved in growth and development, which may explain the phenotypic differences among the fatty and lean swine genotypes.

AB - Iberian pigs are more prone to obesity and metabolic alterations than other lean swine breeds, due to leptin resistance and thrifty genotype, which makes the breed susceptible to changes in amount and composition of diet. This is especially manifested during prenatal development, where nutritional deficiencies in the diet of the mother may modify offspring’s gene expression to cope with postnatal challenging conditions. This work aimed to assess the role of the genotype in the metabolic and transcriptomic changes of pig fetuses derived from undernourished pregnant sows. Pure Iberian sows were inseminated with heterospermic semen from Iberian and Large White boars to obtain IBxIB and IBxLW genotypes. The pregnancy was challenged by diminishingmaternal intake to 50% of requirements during the last 2 thirds of pregnancy. Fifty-one fetuses were obtained at Day 77 of pregnancy and 32 of them (16 from each genotype) were selected at random. Assessment of body traits showed that IBxLW fetuses were heavier and more corpulent than their IBxIB littermates (P < 0.05). Analysis of liver fatty acids (FA) showed that IBxIB had higher level of saturated FA and monounsaturated FA and lower levels of polyunsaturated FA than IBxLW fetuses (P < 0.0001). Liver transcriptome analysis resulted in 249 genes differentially expressed (DE) between the 2 genotypes (q < 0.05 and FC >1.3). Functional annotation of the DE genes revealed a downregulation of biological functions related to growth and development (e.g.: Cell survival, Cell movement, Quantity of connective tissue or Survival of organism) in IBxIB fetuses. Potential transcription factors were assessed with IPA software, which allow the prediction of upstream regulators that may explain the molecular mechanisms responsible of transcriptome differences between genetic types. We highlighted 955 potential upstream regulators, some of them being involvedin growth and development (e.g.: IGF1) and downregulated in IBxIB fetuses. Hence, our results indicate differential regulation of relevant functions and pathways involved in growth and development, which may explain the phenotypic differences among the fatty and lean swine genotypes.

M3 - Abstract

SP - 148

EP - 149

BT - ISAG 2019 Abstract Book

ER -

Garcia Contreras C, Vazquez-Gomez M, Madsen O, Groenen M, Astiz S, Nunez Y et al. Fetal genotype effects on morphomics, fatty acids composition and transcriptomics in swine. In ISAG 2019 Abstract Book: 37th International Society for Animal Genetics Conference, July 7-12, 2019 - Lleida, Spain. 2019. p. 148-149