Fermentation characteristics of various animal tissues by cheetah faecal inoculum

S. Depauw, G. Bosch, A. Becker, M. Hesta, K. Whitehouse-Tedd, W.H. Hendriks, G.P.J. Janssens

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Introduction: Recent studies in the cheetah (Acinonyx jubatus) show a role for undigested animal tissues (e.g. bone, cartilage, hair, skin, feathers) in hindgut fermentation of this strict carnivore (Depauw et al, 2010 a, b). This study aimed to compare the in vitro fermentation kinetics and end-product profiles of different animal-derived substrates, using cheetah faeces as an inoculum. Materials and methods: Fresh faecal samples of eight captive cheetahs were collected within 15 min of defecation, pooled and processed to be used as inoculum. The following raw and homogenised (1mm sieve) substrates were used: rabbit bone, rabbit hair, rabbit skin, whole rabbit, chicken cartilage, beef, beef + bone, beef + cartilage, beef + hair, and beef + skin. Cumulative gas production was continuously recorded over a 72 h period and samples of fermentation liquids were taken at 2, 7, 24 and 72 hours of incubation to determine the end product profile (short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, indole, phenol, p-cresol). Results and discussion: All animal substrates showed an early occurrence of maximum gas production rates (Tmax = 0.1-0.7 h), which confirms our previous findings. Cartilage was highly fermentable and showed the highest gas production as well as SCFA production. Beef exhibited a lower gas production rate than cartilage, and was slower to achieve high SCFA concentrations, which were only detected after 7 hours of incubation, compared to 2 hours for cartilage. Compared to cartilage and beef, whole rabbit, rabbit bone, and skin were low fermentable, and hair exhibited the lowest gas and SCFA production. Fermentation of bone yielded ammonia concentrations that were twice as high as all other substrates. The combination of a fermentable substrate (beef) with low fermentable animal tissue showed no clear interactions. Conclusion: The present data indicate that cartilage and beef are well fermentable substrates for the cheetah, with cartilage being the most fermentable of both. Low fermentable substrates (hair, skin, bone) in homogenised forms did not appear to reduce the fermentation of beef in this in vitro set up. References: available on request.
Original languageEnglish
Title of host publicationProceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011
EditorsC. Castrillo, M.A. Latorre, M. Fondevila, J.A. Guada
Place of PublicationZaragoza
PublisherUniversidad Zaragoza
Pages46-46
Volume15
Publication statusPublished - 2011
Event15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain -
Duration: 14 Sep 201116 Sep 2011

Conference

Conference15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain
Period14/09/1116/09/11

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Acinonyx
Fermentation
Cartilage
Hair
Volatile Fatty Acids
Rabbits
Bone and Bones
Gases
Skin
Ammonia
Red Meat
Feathers
Defecation
Phenol
Feces
Chickens

Cite this

Depauw, S., Bosch, G., Becker, A., Hesta, M., Whitehouse-Tedd, K., Hendriks, W. H., & Janssens, G. P. J. (2011). Fermentation characteristics of various animal tissues by cheetah faecal inoculum. In C. Castrillo, M. A. Latorre, M. Fondevila, & J. A. Guada (Eds.), Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011 (Vol. 15, pp. 46-46). Zaragoza: Universidad Zaragoza.
Depauw, S. ; Bosch, G. ; Becker, A. ; Hesta, M. ; Whitehouse-Tedd, K. ; Hendriks, W.H. ; Janssens, G.P.J. / Fermentation characteristics of various animal tissues by cheetah faecal inoculum. Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011. editor / C. Castrillo ; M.A. Latorre ; M. Fondevila ; J.A. Guada. Vol. 15 Zaragoza : Universidad Zaragoza, 2011. pp. 46-46
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abstract = "Introduction: Recent studies in the cheetah (Acinonyx jubatus) show a role for undigested animal tissues (e.g. bone, cartilage, hair, skin, feathers) in hindgut fermentation of this strict carnivore (Depauw et al, 2010 a, b). This study aimed to compare the in vitro fermentation kinetics and end-product profiles of different animal-derived substrates, using cheetah faeces as an inoculum. Materials and methods: Fresh faecal samples of eight captive cheetahs were collected within 15 min of defecation, pooled and processed to be used as inoculum. The following raw and homogenised (1mm sieve) substrates were used: rabbit bone, rabbit hair, rabbit skin, whole rabbit, chicken cartilage, beef, beef + bone, beef + cartilage, beef + hair, and beef + skin. Cumulative gas production was continuously recorded over a 72 h period and samples of fermentation liquids were taken at 2, 7, 24 and 72 hours of incubation to determine the end product profile (short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, indole, phenol, p-cresol). Results and discussion: All animal substrates showed an early occurrence of maximum gas production rates (Tmax = 0.1-0.7 h), which confirms our previous findings. Cartilage was highly fermentable and showed the highest gas production as well as SCFA production. Beef exhibited a lower gas production rate than cartilage, and was slower to achieve high SCFA concentrations, which were only detected after 7 hours of incubation, compared to 2 hours for cartilage. Compared to cartilage and beef, whole rabbit, rabbit bone, and skin were low fermentable, and hair exhibited the lowest gas and SCFA production. Fermentation of bone yielded ammonia concentrations that were twice as high as all other substrates. The combination of a fermentable substrate (beef) with low fermentable animal tissue showed no clear interactions. Conclusion: The present data indicate that cartilage and beef are well fermentable substrates for the cheetah, with cartilage being the most fermentable of both. Low fermentable substrates (hair, skin, bone) in homogenised forms did not appear to reduce the fermentation of beef in this in vitro set up. References: available on request.",
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Depauw, S, Bosch, G, Becker, A, Hesta, M, Whitehouse-Tedd, K, Hendriks, WH & Janssens, GPJ 2011, Fermentation characteristics of various animal tissues by cheetah faecal inoculum. in C Castrillo, MA Latorre, M Fondevila & JA Guada (eds), Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011. vol. 15, Universidad Zaragoza, Zaragoza, pp. 46-46, 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14/09/11.

Fermentation characteristics of various animal tissues by cheetah faecal inoculum. / Depauw, S.; Bosch, G.; Becker, A.; Hesta, M.; Whitehouse-Tedd, K.; Hendriks, W.H.; Janssens, G.P.J.

Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011. ed. / C. Castrillo; M.A. Latorre; M. Fondevila; J.A. Guada. Vol. 15 Zaragoza : Universidad Zaragoza, 2011. p. 46-46.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Fermentation characteristics of various animal tissues by cheetah faecal inoculum

AU - Depauw, S.

AU - Bosch, G.

AU - Becker, A.

AU - Hesta, M.

AU - Whitehouse-Tedd, K.

AU - Hendriks, W.H.

AU - Janssens, G.P.J.

PY - 2011

Y1 - 2011

N2 - Introduction: Recent studies in the cheetah (Acinonyx jubatus) show a role for undigested animal tissues (e.g. bone, cartilage, hair, skin, feathers) in hindgut fermentation of this strict carnivore (Depauw et al, 2010 a, b). This study aimed to compare the in vitro fermentation kinetics and end-product profiles of different animal-derived substrates, using cheetah faeces as an inoculum. Materials and methods: Fresh faecal samples of eight captive cheetahs were collected within 15 min of defecation, pooled and processed to be used as inoculum. The following raw and homogenised (1mm sieve) substrates were used: rabbit bone, rabbit hair, rabbit skin, whole rabbit, chicken cartilage, beef, beef + bone, beef + cartilage, beef + hair, and beef + skin. Cumulative gas production was continuously recorded over a 72 h period and samples of fermentation liquids were taken at 2, 7, 24 and 72 hours of incubation to determine the end product profile (short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, indole, phenol, p-cresol). Results and discussion: All animal substrates showed an early occurrence of maximum gas production rates (Tmax = 0.1-0.7 h), which confirms our previous findings. Cartilage was highly fermentable and showed the highest gas production as well as SCFA production. Beef exhibited a lower gas production rate than cartilage, and was slower to achieve high SCFA concentrations, which were only detected after 7 hours of incubation, compared to 2 hours for cartilage. Compared to cartilage and beef, whole rabbit, rabbit bone, and skin were low fermentable, and hair exhibited the lowest gas and SCFA production. Fermentation of bone yielded ammonia concentrations that were twice as high as all other substrates. The combination of a fermentable substrate (beef) with low fermentable animal tissue showed no clear interactions. Conclusion: The present data indicate that cartilage and beef are well fermentable substrates for the cheetah, with cartilage being the most fermentable of both. Low fermentable substrates (hair, skin, bone) in homogenised forms did not appear to reduce the fermentation of beef in this in vitro set up. References: available on request.

AB - Introduction: Recent studies in the cheetah (Acinonyx jubatus) show a role for undigested animal tissues (e.g. bone, cartilage, hair, skin, feathers) in hindgut fermentation of this strict carnivore (Depauw et al, 2010 a, b). This study aimed to compare the in vitro fermentation kinetics and end-product profiles of different animal-derived substrates, using cheetah faeces as an inoculum. Materials and methods: Fresh faecal samples of eight captive cheetahs were collected within 15 min of defecation, pooled and processed to be used as inoculum. The following raw and homogenised (1mm sieve) substrates were used: rabbit bone, rabbit hair, rabbit skin, whole rabbit, chicken cartilage, beef, beef + bone, beef + cartilage, beef + hair, and beef + skin. Cumulative gas production was continuously recorded over a 72 h period and samples of fermentation liquids were taken at 2, 7, 24 and 72 hours of incubation to determine the end product profile (short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, indole, phenol, p-cresol). Results and discussion: All animal substrates showed an early occurrence of maximum gas production rates (Tmax = 0.1-0.7 h), which confirms our previous findings. Cartilage was highly fermentable and showed the highest gas production as well as SCFA production. Beef exhibited a lower gas production rate than cartilage, and was slower to achieve high SCFA concentrations, which were only detected after 7 hours of incubation, compared to 2 hours for cartilage. Compared to cartilage and beef, whole rabbit, rabbit bone, and skin were low fermentable, and hair exhibited the lowest gas and SCFA production. Fermentation of bone yielded ammonia concentrations that were twice as high as all other substrates. The combination of a fermentable substrate (beef) with low fermentable animal tissue showed no clear interactions. Conclusion: The present data indicate that cartilage and beef are well fermentable substrates for the cheetah, with cartilage being the most fermentable of both. Low fermentable substrates (hair, skin, bone) in homogenised forms did not appear to reduce the fermentation of beef in this in vitro set up. References: available on request.

M3 - Abstract

VL - 15

SP - 46

EP - 46

BT - Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011

A2 - Castrillo, C.

A2 - Latorre, M.A.

A2 - Fondevila, M.

A2 - Guada, J.A.

PB - Universidad Zaragoza

CY - Zaragoza

ER -

Depauw S, Bosch G, Becker A, Hesta M, Whitehouse-Tedd K, Hendriks WH et al. Fermentation characteristics of various animal tissues by cheetah faecal inoculum. In Castrillo C, Latorre MA, Fondevila M, Guada JA, editors, Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011. Vol. 15. Zaragoza: Universidad Zaragoza. 2011. p. 46-46