Fate of condensed tannin after residing in various compartments of the digestive tract of dairy cows

N.T. Huyen, M. Karonen, J.P. Salminen, I. Mueller-Harvey, W.F. Pellikaan

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Condensed tannins (CT) in the ruminant diets had beneficial effect on utilization of dietary protein, therefore improved wool production, fertility and milk production. Condensed tannins also improved animal welfare and health through prevention of bloat, lowered parasitic burden and decreased ruminal methanogenesis. However, our understanding of the mechanisms of action of CT in the rumen and post-rumen compartments of the ruminant digestive tract is still not yet completely clear. Therefore, the fate of condensed tannins (CT)in the segments of the digestive tract of dairy cows and the fate of CT after in vitro incubation were determined in the current study. The fresh sainfoin (Onobrychis viciifolia cv. Esparcette and Ambra) were cut to 5-10 mm, weighed into nylon bags, then were incubated in the rumen for 2, 4, 7, 10, 24, 48 and 336h. In addition, sainfoin samples in the mobile nylon bags were pre-incubated in the rumen for 12h. Then after, these samples were incubated in the abomasum for 2.5h and from the abomasum through the digestive tract. The CT content were analysed in all samples after incubation in the rumen and in the abomasum and after passing through the digestive tract. In the in vitro study, sainfoin (Ambra) was incubated in 80-mL centrifuge tubes in buffered rumen fluid maintained under anaerobic condition at 39ºC for 1, 2, 4, 8, 12 and 24h. At the end of each incubation time point, the tubes were centrifuged to separate solids from the liquid phase and both fractions were freeze-dried separately and analysed for CT content. The results show that the CT_Proteinand CT_Fiber content were increased, however, the CT_Soluble content was decreased during 24h in vitro incubation. The CT_Protein and CT_Fiber content were increased up to10h incubation in the rumen before they were decreased. The CT_Protein, CT_Fiber and CT_Soluble content were increased in the abomasum while they were reduced again after passage through the lower digestive tract. However, the CT_Soluble was immediately decreased after 2h incubated in the rumen. Based on the results of this current study, it could be concluded that in the entire digestive tract, condensed tannins are always in a form either condensed tannin bound protein or bound fiber. The form of condensed tannin depends on the segment of digestive tract
Original languageEnglish
Title of host publicationWias Annual Conference 2020
Subtitle of host publicationFrontiers in Animal Sciences
PublisherWIAS
Pages79-79
Publication statusPublished - 14 Feb 2020

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    Huyen, N. T., Karonen, M., Salminen, J. P., Mueller-Harvey, I., & Pellikaan, W. F. (2020). Fate of condensed tannin after residing in various compartments of the digestive tract of dairy cows. In Wias Annual Conference 2020: Frontiers in Animal Sciences (pp. 79-79). WIAS. https://edepot.wur.nl/519115