Abstract
A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))
| Original language | English |
|---|---|
| Pages (from-to) | 415-420 |
| Journal | Journal of Microbiological Methods |
| Volume | 57 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 |
Keywords
- n-acylhomoserine lactones
- quorum-sensing signals
- pseudomonas-aeruginosa pao1
- plant-associated bacteria
- agrobacterium-tumefaciens
- variovorax-paradoxus
- virulence
- molecules
- chromatography
- expression
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